肺动脉高压大鼠心肌MMP-2，9和TIMP-1表达的变化  

EXPRESSION CHANGES OF MMP-2,9 AND TIMP-1 IN MYOCARDIAL WITH PULMONARY HYPERTENSION

作　　者：王俊东[1] 杨达宽[1] 李治纲[1] 李树德[1] 

机构地区：[1]云南昆明医学院.昆明 650031

出　　处：《现代预防医学》2011年第6期1106-1108,共3页Modern Preventive Medicine

摘　　要：[目的]研究大鼠肺动脉高压后基质金属蛋白酶(MMPs)和金属蛋白酶组织抑制因子(TIMPs)在左右心室中表达与在心室重构中的作用。[方法]60只SD随机等分为5组,每组12只,1个对照组和4个肺动脉高压实验组(PAH组),分别于4个时间点(MCT注射后第1,2,3,4周)取材。将MCT注射后第1,2,3,4周的4个肺动脉高压实验组(PAH组)分别简称PAH1、PAH2、PAH3和PAH4实验组。采用酶谱法、免疫印迹(western Blotting)、逆转录-聚合酶链反应(RT-PCR),探讨基质金属蛋白酶-2,9(MMP-2,9)蛋白和mRNA的变化规律。[结果](1)右心室MMP-2,9 mRNA在肺动脉高压后1周增加达高峰,以后逐渐下降但到4周时比对照组仍然要高,并且差异有统计学意义(P﹤0.01)。TIMP-1 mRNA在肺动脉高压后从第1周就开始减少,一直到第4周仍然减少,与对照级比较,差异有统计学意义(P﹤0.01)。(2)左心室MMP-2,9蛋白水平在大鼠肺动脉高压后活性稍增强,表达稍增加,而TIMP-1蛋白含量也稍减少,与对照组相比没有统计学差异,并不导致左心室重构。[结论]MMP-2,9蛋白活性与表达在大鼠肺动脉高压右心室心肌中增强,而TIMP-1蛋白活性与表达减少,它们可能与右心室心肌重塑有关。左心室并不重构。[ Objective ] To explore the role of the matrix metaUoproteinases （MMPs） and tissue inhibitor of metallopro- teinase （TIMPs） in the left and right ventricle expression and remodeling on rats with Pulmonary hypertension. [Methods] 60 SD rats were randomly divided into 5groups. 12 BD rats were in each group. There were one control group and 4 experimental pulmonary hypertension （PAH） groups. We took samples at 4 time Points （MCT were injected at the 1, 2, 3 and 4 week）. MCT would be injected at 1, 2, 3 and 4 week after experimental pulmonary hypertension （PAH）, the corresponding experimental groups were regarded as PAHI, PAH2, PAH3 and PAH4 experimental gro,,ps. The activity of MMPs was measured by zymography. The protein and mRNA of MMP-2, -9 and TIMP-1 were determined by Western-Blot an d RT-PCR. [ Results] （1） MMP-2 and 9 mRNA reached peak at 1 week after Pulmonary hypertension, and gradually declined to 4 weeks, but compared with the control level. The experimental group was still higher, there were significant differences significance （P 〈 0.01）. TIMP-1 mRNA reduced in the experimental group from the first to the fourth week. Compared with the control level, there were significant differences significance （P〈 0.01）. （2） In the left ventricle, MMP-2 and 9 Protein activity slighdy increased in rat Pulmonary hypertension, the expression slightly increased, while TIMP-1 Protein content was also slighdy reduced. But there was no significantly difference with the control group, and they could not lead to left ventrieular remodeling. [ ConclUsion] MMP-2, 9 Protein activity and its expression increased in rat right ventricle with Pulmonary hypertension. But TIMP-I Protein activity and expression decreased in rat right ventricle with Pulmonary hypertension. They may be related to right myocardial re- modeling. Not left ventrieular remodeling.

关 键 词：肺动脉高压:心室重建 基质金属蛋白酶 聚合酶链反应 

分 类 号：R543.2[医药卫生—心血管疾病]