RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因用于慢性日本血吸虫病诊断的研究  

Study on RT-PCR-amplified Sj26 and Sj32 and Sj14-3-3 antigen genes for use in the diagnosis of chronic schistosomiasis japonica

作　　者：蔡世飞[1] 李文桂[1] 王敏[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国病原生物学杂志》2011年第2期133-135,共3页Journal of Pathogen Biology

基　　金：重庆市科委地方病重大专项基金(No.2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的探讨RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因用于慢性日本血吸虫病诊断的价值。方法从慢性日本血吸虫病患者血清中提取总RNA,用RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因,以1.2%琼脂糖凝胶电泳进行鉴定,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型包虫病、囊型包虫病、乙型肝炎、肺结核患者血清和健康人血清作为对照。结果 RT-PCR扩增出约400 bp的Sj14-3-3抗原编码基因片段,但未扩增出676 bp的Sj26和1 270bp的Sj32抗原编码基因片段。对照组呈阴性反应。结论 RT-PCR扩增Sj14-3-3抗原编码基因可用于慢性日本血吸虫病的基因诊断。Objective To investigate the diagnostic value of antigen genes Sj26,Sj32,and Sj14-3-3 amplified by RT-PCR for diagnosis of chronic schistosomiasis japonica.Methods Total RNA was extracted from sera of patients with chronic schistosomiasis japonica.Antigen genes Sj26,Sj32,and Sj14-3-3 were amplified by RT-PCR and identified by 1.2% agarose gel electrophoresis.The sera of patients with clonorchiasis sinensis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or pulmonary tuberculosis and sera of healthy people served as a control.Results Sj14-3-3 antigen gene fragments of about 400 bp were obtained from total RNA by RT-PCR,but Sj26 and Sj32 antigen gene fragments of 676 bp and 1270 bp,respectively,were not obtained.Normal control groups were negative.Conclusion RT-PCR-amplified Sj14-3-3 antigen genes can be used in the genetic diagnosis of chronic schistosomiasis japonicum.

关 键 词：日本血吸虫病 基因诊断 逆转录-聚合酶链反应 

分 类 号：R532.21[医药卫生—内科学]