化瘀通络方对局灶性脑缺血大鼠再灌注(MCAO)后脑组织氧自由基和NOS表达的影响  

Effect of Huayu Tongluo Fang on the Expression of ROS and NOS in Brain Tissue During the Reperfusion After Middle Cerebral Artery Occlusion in Rats

作　　者：邱召娟[1] 朱萱萱[1] 达庆国[1] 吴旭彤[1] 朱吾元[2] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学,江苏南京210029

出　　处：《中华中医药学刊》2011年第3期509-511,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省中药药效与安全性评价重点实验室资助项目(P09017);江苏省中医药管理局资助项目(LZ09048)

摘　　要：目的:观察化瘀通络方治疗大鼠局灶性脑缺血-再灌注损伤对脑组织自由基及一氧化氮合成酶(NOS)和iNOS变化的影响。方法:制备大鼠局灶性脑缺血再灌注损伤模型,给予化瘀通络方治疗后观察丙二醛(MDA)、超氧化物歧化酶(SOD)及一氧化氮合成酶(NOS)和诱导型一氧化氮合成酶(iNOS)的变化。结果:化瘀通络方可显著降低MDA含量以及iNOS和NOS活性,SOD含量升高。结论:在脑缺血再灌注损伤后,化瘀通络方能抑制脑缺血再灌注后脂质过氧化反应,促进自由基清除,对抗自由基损伤,提高脑组织自身抗氧化能力,降低NOS和iNOS介导脑缺血-再灌注时的神经损伤,保护脑细胞。Objective：To observe the effect of huayutongluofang on expression of ROS,NOS and iNOS during the reperfusion after middle cerebral artery occlusion in rats.Methods：The cerebral IR rats model were established by middle cerebral artery occlusion.Treated the cerebral IR rats with huayutongluofang,then observing the changes of MDA,SOD,NOS and iNOS in brain tissue.Results：Huayutongluofang could reduce the expression of MDA,iNOS and NOS in brain tissue.While,after huayutongluofang treatment,the SOD level in brain tissue was increased.Conclusions：During the reperfusion after middle cerebral artery occlusion,huayutongluofang can restrain the Lipid Peroxidation,promote removal of oxygen free radicals,compete oxygen-free radical injury.While,runhoukaiyinkeli could reduce the possibility of nerve damage,protect neural cells from injured.

关 键 词：化瘀通络方 脑缺血-再灌注损伤模型 SOD MDA NOS INOS 

分 类 号：R285.5[医药卫生—中药学]