地黄益智方对D-半乳糖衰老模型大鼠脑组织SA-β-gal活性的影响  

Effects of Dihuang-Yi-Zhi Formula on Activities of Senescence-associated β-galactosidase in D-galactose Induced Senile Rats

作　　者：安红梅[1] 史云峰[1] 胡兵[1] 史秀峰[1] 许丽雯[1] 顾明昌[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中华中医药学刊》2011年第3期547-549,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：上海市教委创新项目(10YZ58);上海市科委中药现代化项目(10DZ1971200);国家自然科学基金项目(81072789)

摘　　要：目的:探讨抗衰老中药方剂地黄益智方对D-半乳糖衰老模型大鼠脑组织SA-β-gal表达的影响。方法:D-半乳糖法诱导大鼠衰老,地黄益智方中药治疗,组织化学法检测大脑衰老标志产物SA-β-gal活性。结果:D-半乳糖作用后大鼠脑组织呈现SA-β-gal阳性,地黄益智方能抑制衰老大鼠脑组织SA-β-gal活性。结论:地黄益智方可抑制衰老大鼠脑组织细胞衰老,提示地黄益智方可延缓脑衰老。Objective：To observe the effects of Dihuang-Yi-Zhi formula,an anti-age Herbal Prescription,on activities of senescence-associated β-galactosidase in D-galactose induced senile rats.Methods：Senile rats were induced by D-galactose,and treated with three different doses of Dihuang-Yi-Zhi formula.Senescence-associated β-galactosidase（SA-β-gal）,a marker of cell senescence,was detected by histochemistry.Results：SA-β-gal activities were detected in brain tissue of senile rats induced by D-galactose.Treatment with Dihuang-Yi-Zhi formula resulted in inhibition of SA-β-gal activities.Conclusion：Dihuang-Yi-Zhi formula was effective in inhibiting cell senescence in brain tissue of D-galactose induced senile rats,which suggests Dihuang-Yi-Zhi formula may delay brain aging.

关 键 词：地黄益智方 脑衰老 细胞衰老 

分 类 号：R285.5[医药卫生—中药学]