反相高效液相色谱法测定尿中α-萘酚、β-萘酚、对硝基酚和间硝基酚  

Determination of α-Naphthol,β-Naphthol,p-Nitrophenol and m-Nitrophenol in Human Urine by Reversed Phase High Performance Liquid Chromatography

作　　者：黎俊宏[1,2] 李贵荣[2] 唐宏兵[1] 徐晓怡[1] 欧阳运富[1] 王永生[2] 杨红梅[3,2] 

机构地区：[1]常州市疾病预防控制中心,江苏省常州市泰山路203号213022 [2]南华大学公共卫生学院,湖南省衡阳市常胜西路28号421001 [3]常州市中心血站,江苏省常州市劳动中路8号213004

出　　处：《光谱实验室》2011年第2期782-786,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省自然科学基金(03JJY3030);湖南省科学技术基金(2008FJ3083)

摘　　要：利用反相高效液相色谱法建立了同时检测人尿样中的α-萘酚、β-萘酚、对硝基酚和间硝基酚的新方法。采用DiamonsilTMC18(150mm×4.6mm,5μm)色谱柱分离,以V(甲醇)∶V(乙酸铵缓冲液)=58∶42为流动相,流速为0.75mL/min,柱温为35℃,于波长280nm处检测。当α-萘酚、β-萘酚、对硝基酚和间硝基酚的浓度分别在0.120—19.44、0.106—19.44、0.479—27.80μg/mL和0.439—27.80μg/mL范围内与峰面积呈良好线性关系,RSD分别为3.7%—4.3%、2.4%—3.6%、2.7%—4.9%和2.9%—3.6%(n=5)。本法用于尿中4种物质的同时测定,回收率分别为98.8%、103.8%、97.5%和105.0%,结果满意。A novel method for the determination of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol in human urine was established by RP-HPLC.The four objects were separated on DiamonsilTMC18（150mm×4.6mm,5μm） using methanol-ammonium acetate buffer solution（V∶V=58∶42） as mobile phase and the flow rate was 0.75mL/min.They were detected at 280nm when the column temperature was 35℃.The calibration curves of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol showed good linearities in the ranges of 0.120—19.44,0.106—19.44,0.479—27.80μg/mL and 0.439—27.80μg/mL,respectively.The RSD were 3.7%—4.3%,2.4%—3.6%,2.7%—4.9%,2.9%—3.6%（n=5）,respectively.The proposed method was used for the determination of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol in human urine.The recoveries of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol were 98.8%,103.8%,97.5% and 105.0%,respectively.The results are satisfactory.

关 键 词：反相高效液相色谱 萘酚 硝基酚 尿样 

分 类 号：O657.72[理学—分析化学]