RP-HPLC测定巴豆种仁中佛波醇的含量  

Determination of Phorbol in the Seeds of Croton Tiglium Linn by RP-HPLC

作　　者：郭虎[1] 彭飞[1] 马廉举[1] 姜展[1] 刘新[1,2] 

机构地区：[1]重庆医科大学药学院重庆市渝中区医学院 [2]重庆高校药物工程研究中心,重庆市400016

出　　处：《光谱实验室》2011年第2期801-805,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立巴豆(Croton tiglium Linn)种仁中佛波醇的HPLC含量测定方法。采用岛津LC-2010型液相色谱仪,Diamonsil C18分析柱(250mm×4.6mm,5μm),流动相:乙腈-水(梯度洗脱),检测波长:234nm,流速:1mL/min,柱温:25℃。在选定色谱条件下佛波醇浓度在6.096—101.600μg/mL范围内与峰面积的线性关系良好,r=0.9999,平均加样回收率为96.40%,RSD为1.03%。本实验建立了RP-HPLC测定巴豆种仁中佛波醇的含量,为佛波醇的进一步研究提供了一种手段。The determination of phorbol in the seeds of Croton tiglium linn by HPLC was investigated.Shimadzu LC-2010 HPLC,Diamonsil C18 column（250mm×4.6mm,5μm） were uesd,and the mobile phase was consisted of acetonitrile-water（gradient elution） with the flow rate of 1mL/min.The detection wavelength was 234nm,and the column temperature was 25℃.There was a good linear relationship of phorbol in the range of 6.096—101.600μg/mL（r=0.9999）,while the average standard recovery was 96.40% with RSD of 1.03%.The method is established for determination of phorbol in the seeds of Croton tiglium linn by RP-HPLC,and provides a means for future study.

关 键 词：佛波醇 巴豆 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]