弯孢马利亚霉SIIA-F12361产生的新代谢产物研究  

A novel secondary metabolite from Mariannaea campospora SIIA-F12361

作　　者：王辂[1] 郭义东[1] 褚以文[1] 

机构地区：[1]抗生素研究与再评价四川省重点实验室中国医药集团总公司四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2011年第3期179-182,186,共5页Chinese Journal of Antibiotics

基　　金：科技基础条件平台项目(No.2005DKA21203);天然药物及仿生药物国家重点实验室(北京大学)开放基金(20080214);抗生素研究与再评价四川省重点实验室开放基金(No.2009JC012)

摘　　要：目的对海洋生境来源菌株SIIA-F12361进行分类学鉴定以及次级代谢产物的化学研究。方法采用形态学与18S rDNA序列分析对菌株进行分类学鉴定。通过硅胶柱层析、LH-20分子筛层析和反相制备HPLC诸方法,对新化合物进行分离纯化,并根据高分辨质谱、紫外、红外光谱和核磁共振数据对化合物SIIA-C12361进行结构鉴定。结果鉴定菌株SIIA-F12361为弯孢马利亚霉,其产生的新次级代谢产物属于倍半萜烯内酯类化合物。结论弯孢马利亚霉SIIA-F12361产生一新倍半萜烯内酯化合物。Objective To investigate the taxonomy of a fungal strain SIIA-F12361 isolated from marine environment, as well as its secondary metabolites. Methods The strain SIIA-F12361 was identified by morphology and 18S rDNA sequence analysis. The compound SIIA-C12361 was isolated by using silica gel column chromatography, LH-20 chromatography and preparative HPLC. The planner structure of SIIA-C12361 was determined through extensive analysis of MS, UV, IR and various NMR spectroscopic data. Results The isolate of SIIA-F12361 was identified as Mariannaea campospora. Additionally, one new sesquiterpene lactone SIIA- C12361 was purified and structural elucidated. Conclusion Mariannaea campospora SIIA-F12361 produces a new sesquiterpene lactone.

关 键 词：弯孢马利亚霉 倍半萜烯内酯 分离纯化 结构解析 

分 类 号：Q936[生物学—微生物学]