凝胶色谱法测定头孢地尼的聚合物  

Determination of polymer in cefdinir by gel filtration chromatography

作　　者：谢赞[1] 陈玉洁[1] 李坤[1] 何晓燕[1] 史颖[1] 

机构地区：[1]石药集团中奇制药技术(石家庄)有限公司,石家庄050051

出　　处：《中国抗生素杂志》2011年第3期214-216,共3页Chinese Journal of Antibiotics

基　　金：国家科技支撑计划(NO.2007BAI26B04)

摘　　要：目的建立凝胶色谱法测定头孢地尼中聚合物的方法。方法色谱柱为Sephadex G-10柱（15．0mm×300mm），流动相A为pH7．0的0．1mol／L磷酸盐缓冲液，流动相B为水。流速为2．0mL／min，检测波长为254nm，进样量为200uL。结果该方法在4．1～20．3mg／mL范围内与峰面积呈良好的线性关系（r=0．9999），头孢地尼中聚合物含量小于0．1％。结论该方法简便、准确、灵敏度高，适用于头孢地尼聚合物的控制。Objective To establish a gel filtration chromatography method for determination of the polymer in cefdinir. Methods The chromatographic column was Sephadex G-10 （15.0mm×300mm）; mobile phase A, 0.1mol/L phosphate buffer （pH7.0）; B, water; the flow rate was 2.0mL/min; the detection wavelength was 254nm and the injection volume was 200uL. Results The good linearity was obtained between peak area and concentration ranging from 4.1-20.3mg/mL, the content of polymer in cefdinir is less than 0.1%. Conclusion The method is simple, accurate and sensitive for the control of the polymer in cefdinir.

关 键 词：头孢地尼 聚合物 凝胶色谱法 

分 类 号：R978.11[医药卫生—药品]