川贝母新资源太白贝母中生物碱类成分含量测定  

Determination of Main Alkaloids in Fritillaria taipaiensis by HPLC-ELSD

作　　者：段宝忠[1] 王丽芝[1] 黄林芳[1] 魏大华 卢其福 

机构地区：[1]中国医学科学院-北京协和医学院药用植物研究所,北京100193 [2]广州潘高寿药业股份有限公司,广州511490

出　　处：《中国药学杂志》2011年第5期382-384,共3页Chinese Pharmaceutical Journal

基　　金：科技部十一五支撑计划项目(2006BAI09B05-3);中医药行业专项基金(200707007);中医药行业专项基金(200807020)

摘　　要：目的利用高效液相色谱法测定太白贝母中的生物碱成分。方法采用Agilent Extend C18柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.02%三乙胺水溶液,梯度洗脱,流速为1.0 mL.min^-1,漂移管温度90℃;载气体积流量2.3 L.min-1,增益值2,进样量10μL;柱温25℃。结果贝母辛在28.75-690.0μg.mL^-1,贝母素乙在27.37-560.6μg.mL^-1,贝母素甲在25.75-527.0μg.mL^-1与峰面积的对数值呈线性关系,平均回收率分别为99.4%（n=6）,RSD为1.10%;99.7%（n=6）,RSD为3.61%;100.3%（n=6）,RSD为2.82%。结论太白贝母中的生物碱成分与川贝母非常相似,为其作为川贝母来源的合理性奠定了基础。OBJECTIVE To develop a HPLC method for determining the contents of peimisine,imperialine and peiminine in Fritillaria taipaiensis.METHODS The analyses were performed on an Agilent Extend-C18 column（4.6 mm×250 mm,5 μm）.The investigated compounds were separated with gradient mobile phase consisting of methanol-water-triethylamine at the flow of 1.0 mL·min-1.The temperature for the detector drift tube was set at 90 ℃ and the nitrogen flow-rate was 2.3 L·min-1,the gain in ELSD was set at 2 in order to obtain the best sensitivity,and the injection volume was 10 μL.RESULTS The investigated compounds including peimisine,peiminine and peimine had good linearity（r2≥0.9991） within the tested ranges.The average recoveries were 99.4%,99.7% and 100.3% with RSD≤3.61%.CONCLUSION The developed method can be applied to control the quality of the F.taipaiensis with a good accuracy,sensitivity and repeatability.

关 键 词：太白贝母 生物碱 高效液相色谱-蒸发光检测法 

分 类 号：R917[医药卫生—药物分析学]