具酯酶活性的温敏型分子印迹微凝胶  

Temperature-sensitive molecularly imprinted microgels with esterase activity

作　　者：王红飞[1,2] 杨浩 张黎明[1,2] 

机构地区：[1]新型聚合物材料设计合成与应用广东省高校重点实验室 [2]中山大学化学与化学工程学院,广州510275 [2]聚合物复合材料及功能材料教育部重点实验室 [4]中山大学化学与化学工程学院,广州510275

出　　处：《中国科学：化学》2011年第3期524-530,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(21074152,20874116,20676155,J0730420);教育部高等学校博士点基金(20090171110023);广东省自然科学基金(9151027501000105,8151027501000004)资助

摘　　要：以双醛葡聚糖-组氨酸偶连物(PAD-His)为功能大单体、过渡态类似物p-硝基苯磷酸酯(NPP)为模板分子、Co2+为中心离子,采用油包水反相乳液法首次制得具有酯酶活性的温敏型分子印迹微凝胶(MIGs).催化水解实验表明,MIGs催化活性受模板分子用量的影响,并可通过温度进行有效调控.MIGs催化p-硝基乙酸苯酯(NPA)水解反应行为可用Michaelis-Menten方程进行描述,其最大催化水解反应速率和Michaelis-Menten常数分别为2.04×10-8mol/h和2.2×10-5mol/L,且具有较好的催化选择性.Temperature-sensitive molecularly imprinted microgels （MIGs） exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate （PAD-His） as the functional macromonomer and p-nitrophenyl phosphate （NPP） as the stable transition state analogue （TSA） as well as Co2＋ as the coordination center. The catalytic activity of MIGs was greatly influenced by the template amount, and could be modulated by temperature. The hydrolysis kinetics of p-nitrophenyl acetate （NPA） in the presence of MIGs could be described by the Michaelis-Menten equation. The Michaelis-Menten constant and maximium velocity were found to be 2.2×10-5 mol/L and 2.04×10-8 mol/h, respectively. In addition, the MIGs were found to have a high catalytic selectivity to NPA.

关 键 词：分子印迹 微凝胶 酯酶 温度敏感葡聚糖 

分 类 号：O631.3[理学—高分子化学]