多功能放线菌Act12对土传病原真菌的拮抗性及其鉴定  

Antagonistic effect of multifunctional actinomycete strain Act12 on soil-borne pathogenic fungi and its identification

作　　者：赵娟[1] 薛泉宏[2] 王玲娜[1] 段春梅[2] 薛磊[1] 毛宁[2] 

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100 [2]西北农林科技大学资源环境学院,杨凌712100

出　　处：《中国生态农业学报》2011年第2期394-398,共5页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金重点项目(30630054)资助

摘　　要：采用琼脂块法及抑菌试验,研究了多功能放线菌Act12对6种常见土传病原真菌的拮抗作用;采用形态特征、生理生化特性及16 S rDNA序列分析,确定了Act12的分类地位。结果表明,Act12对6种供试土传病原真菌具有不同程度的拮抗作用,其中,对木贼镰刀菌(Fusarium equiseti)和尖孢镰刀菌西瓜专化型(F.ox-ysporum f.sp.niveum)的拮抗圈直径分别达到20.5 mm和18.4 mm;培养48 h,Act12无菌发酵滤液对木贼镰刀菌的抑菌率达到83.2%。菌株显微形态、培养特征、生理生化特性及16 S rDNA序列分析结果表明,多功能放线菌Act12为链霉菌属的密旋链霉菌(Streptomyces pactum)。Antagonistic effects of multifunctional actinomycete strain Act12 on six soil-borne pathogenic fungi were determined using agar block method and inhibitory test.The strain Act12 was identified by analyses on its morphological character,physiological and biochemical characteristics,as well as 16 S rDNA sequence.The results showed varying degrees of Act12 antagonism against all tested six soil-borne pathogenic fungi.The widths of inhibitory zones against Fusarium equiseti and F.oxysporum f.sp.niveum were 20.5 mm and 18.4 mm,respectively.The rate of bacteriostasis of Act12 axenic fermentation filtrate against F.equiseti after 48 h cul-tivation was 83.2%.Based on micro-morphology,and cultural,physiological and biochemical characteristics,as well as 16 S rDNA sequence analysis,Act12 strain was identified as Streptomyces pactum.

关 键 词：放线菌 连作障碍 密旋链霉菌 拮抗性 微生物修复 抑菌试验 

分 类 号：S432.44[农业科学—植物病理学] Q939.132[农业科学—农业昆虫与害虫防治]