RP-HPLC法测定Beagle犬血浆中四环素  

HPLC determination of tetracycline in Bealge dog plasma

作　　者：张煦喆[1] 刘有平[1] 关锋[1] 王鑫[1] 邸欣[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2011年第3期509-512,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定Beagle犬血浆中四环素的RP-HPLC分析方法。方法:Beagle犬血浆样品经0.01 mol·L-1草酸酸化、乙腈沉淀蛋白处理后,采用Diamonsil C18柱(200 mm×4.6 mm,5μm)分离,流动相为乙腈-0.01 mol·L-1草酸溶液(15∶85),流速为0.8 mL·min-1,检测波长为350 nm,内标为土霉素。结果:测定血浆中四环素的线性范围为0.2～10μg·mL-1;日内与日间精密度均小于14.5%,准确度(RE)在$5.3%以内。结论:本方法简便、灵敏、准确,适用于Beagle犬血浆中四环素的分析测定。Objective：To develop an RP-HPLC method for the determination of tetracycline in Beagle dog plasma.Methods：After acidification with oxalic acid,plasma samples were precipitated with acetonitrile.Chromatographic separation was carried out on a Diamonsil C18 column（200 mm × 4.6 mm,5 μm）using a mobile phase of acetonitrile-0.01 mol·L-1 oxalic acid（15∶85）.The flow rate was 0.8 mL·min-1 and the detection wavelength was 350 nm.Oxytetracycline was used as the internal standard.Results：The calibration curve for tetracycline was linear over the concentration range of 0.2-10 μg·mL-1.The intra-and inter-day precision was less than 14.5% and the accuracy was within ±5.3%.Conclusion：The method is simple,sensitive,accurate and suitable for the preclinical pharmacokinetic studies of tetracycline in dog plasma.

关 键 词：四环素 RP-HPLC法 BEAGLE犬 血浆 

分 类 号：R917[医药卫生—药物分析学]