不同治法方药对激素性股骨头坏死鸡成骨相关因子的影响  

Effect of different treating principles and formulas on expression of osteogenic factors in steroid-induced osteonecrosis of femoral head of chichen

作　　者：孔祥英[1] 万蓉[1] 李莉[2] 林诗富[2] 郭炜[1] 林娜[1] 孔焕宇[2] 陈卫衡[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院望京医院,北京100102

出　　处：《中国中药杂志》2011年第5期614-617,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30472131)

摘　　要：目的:通过观察健脾化痰、活血通络(健脾)与补肾壮骨、活血通络(补肾)2种不同治法方药对激素性股骨头坏死鸡成骨因子骨形成蛋白(BMP)2、转化生长因子(TGF)β1及其下游蛋白Smads的影响,比较探讨其防治股骨头坏死的作用机制。方法:48只来杭鸡经胸肌注射甲基氢化泼尼松建立激素性股骨头坏死模型后,随机分为模型组、健脾组和补肾组,另16只来杭鸡设为正常对照组,4个实验组在造模同时分别灌胃生理盐水和相应中药。给药8周和16周后取双侧股骨头,免疫组织化学法检测BMP2,TGFβ1和Smad4,7蛋白表达。结果:与正常组相比,模型组股骨头BMP2,TGFβ1和Smad4阳性细胞计数显著减少、Smad7明显增加;与模型组相比,健脾组8周时BMP2,TGFβ1和Smad4阳性细胞计数显著增多、Smad7明显减少,与补肾组在16周时的作用相类似。结论:健脾与补肾2种不同治法方药均能通过调节股骨头内成骨因子BMP2,TGFβ1和Smad4,7蛋白表达来促进股骨头的修复,防治股骨头坏死;健脾方药比补肾方药起效时间更早。Objective：To investigate the effect of two treating principles and formulas,which are named ′invigorating spleen to remove phlem and promoting blood circulation to remove meridian obstruction′（Jianpi） and ′invigorate the kidney and promoting blood circulation to remove meridian obstruction′（Bushen）,on the expression of osteogenic factors in steroid-induced osteonecrosis of the femoral head（SONFH）,such as bone morphogenetic protein 2（BMP2） and transforming growth factor β1（TGFβ1） and Smads,as well as to explore and compare their mechanisms of prevention and treatment of SONFH.Method：Animal model of SONFH was established by injection with methylprednisolone in chest muscle on chickens.48 SONFH chickens were randomly assigned to model,Jianpi and Bushen group.Another 16 normal chickens served as control group.At the 8th and 16th week,the expression of BMP2,TGFβ1,Smad4 and Smad7 of bilateral femoral heads were detected with immunohistochemistry.Result：The expression of BMP2,TGFβ1 and Smad4 decreased,and Smad7 increased significantly in model group compared with control group.The expression of BMP2,TGFβ1,Smad4 increased and Smad7 decreased significantly in Jianpi group at the 8th week compared with model group,and the same changes in Bushen group at the16th week.Conclusion：Both Jianpi and Bushen formulas exerted preventive and therapeutic activity on SONFH through regulating the expression of BMP2,TGFβ1,Smad4 and Smad7 to promote bone repair.Notably Jianpi formula took effect earlier than Bushen formula.

关 键 词：股骨头坏死 BMP2 TGFΒ1 SMADS 

分 类 号：R681.8[医药卫生—骨科学]