全蝎蜈蚣对CIA大鼠外周血和小肠黏膜T细胞亚群的影响  

Effect of oral scorpio and scolopendra powder on T-cell subsets in peripheral blood and intestine from rats with collagen induced arthritis

作　　者：程绍民[1] 赵海梅[2] 左志琴[2] 王艳辉[3] 王跃生[4] 刘端勇[2] 

机构地区：[1]江西中医学院基础医学院,江西南昌330004 [2]江西中医学院科技学院,江西南昌330025 [3]南昌大学抚州医学分院,江西抚州334000 [4]中药固体制剂制造技术国家工程研究中心,江西南昌330006

出　　处：《中国中药杂志》2011年第5期633-635,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30860377);江西省自然科学基金项目(2009GZY0118);江西省卫生厅科技计划项目(200920520;20092052)

摘　　要：目的:观察全蝎蜈蚣对CIA大鼠外周血和小肠黏膜T细胞亚群的影响。方法:60只Wistar大鼠除正常组外均复制胶原免疫性关节炎后,并随机分成模型组、全蝎蜈蚣高、中、低剂量组和Ⅱ型胶原蛋白治疗组,给药40 d,采用流式细胞术检测大鼠外周血和小肠黏膜T细胞亚群水平。结果:与正常组比较,模型组大鼠外周血和PP结T淋巴细胞均表现为CD4+T细胞明显升高或有升高趋势,CD4+/CD8+则在外周血升高而在PP结下降(P<0.05或P<0.01)。与模型组比较,所有治疗组对CD3+T淋巴细胞作用不明显,全蝎蜈蚣各剂量组均可明显下调PP结CD4+,CD8+T细胞水平,升高CD4+/CD8+水平(P<0.05或P<0.01),同时外周血方面高、中剂量组CD4+T淋巴细胞水平及CD4+/CD8+显著下降(P<0.05或P<0.01),但对CD8+T淋巴细胞作用不明显。结论:全蝎蜈蚣治疗类风湿性关节炎的可能途径是调节CIA大鼠外周血和肠黏膜局部T淋巴细胞平衡。Objective：To observe effect of oral scorpio and scolopendra powder on T-cell subsets in peripheral blood and intestine from rats with collagen induced arthritis（CIA）.Method：60 rats were randomly divided into 6 groups：normal control group,model control group,low-dose scorpio and scolopendra group,middle-dose scorpio and scolopendra group,high-dose scorpio and scolopendra group,and type Ⅱcollagen group.Rat′s rheumatoid arthritis was induced by collagen Ⅱ（CⅡ）.Level of T-cell subsets from peripheral blood and intestine was measured by flow cytometry.Result：CD4＋T cellular level was obviously increased（P0.05 or P0.01） or kept increased tendency in peripheral blood and intestine from the model group compared with that of the normal group,while the ratio of CD4＋/CD8＋ in intestine was obviously descent but the contrary in peripheral blood（P0.05 or P0.01）.CD4＋,CD8＋ T cellular level in intestine were obviously descent and the ratio of CD4＋/CD8＋ increased in all treated groups when compared with in the model group（P0.05 or P0.01）.However,CD4＋ T cellular level and the ratio of CD4＋/CD8＋ in peripheral blood were remarkablely decreased.Conclusion：The mechanism that scorpio and scolopendra could treat rat′s rheumatoid arthritis may be regulating balance of T-lymphocyte subsets in peripheral blood and intestine.

关 键 词：类风湿性关节炎 全蝎 蜈蚣 T细胞亚群 

分 类 号：R285.5[医药卫生—中药学]