曲古抑菌素A上调CD_4^+T细胞叉头框蛋白3基因表达  

Trichostain A on FOXP3 gene expression in CD_4^+T cells

作　　者：侯粲[1] 蔺怡[1] 李一君[1] 吴碧琛[1] 周智广[1] 

机构地区：[1]中南大学湘雅二医院内分泌科(代谢内分泌研究所糖尿病免疫学教育部重点实验室),湖南长沙410011

出　　处：《中国现代医学杂志》2011年第2期194-196,共3页China Journal of Modern Medicine

基　　金：湖南省高校科技创新团队支持计划资助

摘　　要：目的探讨组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)干预对正常人CD4+T细胞FOXP3表达的影响。方法采用密度梯度离心法分离正常人外周血单个核细胞,免疫磁珠分离CD4+T细胞,分别给予TSA处理培养相应时间后收集细胞,RT-PCR法分析TSA干预对正常CD4+T细胞FOXP3 mRNA表达影响。结果 100nmol/L TSA干预48h可上调正常CD4+T细胞FOXP3表达。结论 TSA干预能上调CD4+T细胞FOXP3表达。【Objective】 To investigate whether the histone deacetylase inhibitor,Trichostatin A（TSA） interfere with FOXP3 expression in normal CD4＋ T cells.【Methods】 Peripheral blood mononuclear cells（PBMC） isolated from blood of healthy volunteers were prepared using the Ficoll-Hypaque method.CD4＋ T cells were isolated using magnetic beads.CD4＋ T cells were treated with different concentrations of TSA.Cells were collected after appropriate time,and the expression of FOXP3 mRNA was analyzed by RT-PCR.【Results】 100 nmol/L TSA intervention increased FOXP3 expression in normal CD4＋ T cells after 48 hours treatment.【Conclusion】 TSA treatment increased FOXP3 expression in normal CD4＋ T cells.

关 键 词：曲古抑菌素A CD4+T细胞 FOXP3 

分 类 号：R34[医药卫生—基础医学]