“嗅三针”对阿尔茨海默病大鼠海马Bcl-2和Bax表达的干预效应  

Effect of "Xiusanzhen" on Expression of Hippocampal Bcl-2 and Bax Proteins in Alzheimer Disease Rats

作　　者：刘智斌[1] 牛文民[1] 杨晓航[2] 牛晓梅[2] 王渊[2] 

机构地区：[1]陕西中医学院针灸推拿系,咸阳712046 [2]陕西中医学院附属医院,咸阳712020

出　　处：《针刺研究》2011年第1期7-11,共5页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.30973792)

摘　　要：目的:探讨"嗅三针"通过嗅觉通路对阿尔茨海默病(AD)模型大鼠海马神经元细胞凋亡的影响,以阐明其治疗AD的作用机制。方法:成年SD雄性大鼠40只,随机分为正常对照组、AD模型组、嗅神经切断嗅三针组、嗅三针组,每组10只。β-淀粉样蛋白(Aβ)1-40肽段注射法制作AD大鼠模型,嗅神经切断术造大鼠嗅觉通路阻断模型。"印堂"穴及两侧"迎香"穴交替电针刺激行"嗅三针"治疗,每次10 min,每周治疗5次,共治疗6周。免疫组化法检测海马Bcl-2和Bax表达。结果:AD模型组较正常对照组Bcl-2阳性细胞减少(P<0.05),Bax阳性细胞表达增多(P<0.01);嗅三针组较AD模型组Bcl-2阳性细胞明显增多(P<0.01),Bax阳性细胞显著减少(P<0.01);嗅神经切断嗅三针组与AD模型组比较Bcl-2、Bax阳性细胞变化不明显(P>0.05)。结论:"嗅三针"能够调节海马Bcl-2和Bax的表达,其治疗效应的发挥依赖于嗅觉传导通路的完整性。Objective To observe the effect of electroacupuncture （EA） of ＂Xiusanzhen＂[3 acupoints, i.e. , bilateral ＂Yingxiang＂ （L120） and ＂Yintang＂ （EX-HN 3）] on expression of B-cell lymphoma/leukemia-2 （Bcl-2, an antiapoptosis protein） and Bax （a protein for promoting apoptosis） in the hippocampus in Alzheimer disease（AD）rats, so as to explore its clinical mechanisms underlying relieving AD. Methods A total of 40 Sprague Dawley rats were randomly divided into normal controI,AD model, EA and olfactory nerve transaction（ONT） ＋ EA groups, with 10 rats in each. AD model was established by injection of [3-amyloid （Aβ）1-40 （2μL containing 10 Lug） into the hippocampus （Bregma： 3.5 mm, L： 2.0 mm, H： -2.8 mm below dura）, and the ON was sectioned by using a fine needle. EA （80-100 Hz, 1-3 mA） was applied to bilateral LI20 and EX-HN 3 for 10 min, once daily （except Saturdays and Sundays） for 6 weeks. The expression of Bcl-2 and Bax of hippocampus was measured by immunohistochemistory. Results Compared with the normal control group, the expression of Bcl-2 of hippocampus was significantly lower in the AD model group（P〈0.05）, and that of Bax in AD model group was much higher（P〈0.01）. In comparison with the model group, the expression of hippocampal Bcl-2 was up-regulated significantly（ P〈0.01 ）, and that of hippocampal Bax protein down- regulated considerably in the EA group（P〈0.01）. No significant differences were found between AD model and ONT＋ EA groups in the expression of hippocampal Bcl-2 and Bax proteins （P〈0.05）, suggesting a necessary premise condition for EA in regulating hippocampal Bcl-2 and Bax expression. Conclusion ＂Xiusanzhen＂ can regulate the expression of hippocampal Bcl-2 and Bax proteins in AD rats, which may contribute to its clinical effect in relieving AD, and the therapeutic effect depends on the integrity of the olfactory nerve pathway.

关 键 词：阿尔茨海默病 电针 嗅三针 海马 嗅神经通路 BCL-2 BAX 

分 类 号：R245.97[医药卫生—针灸推拿学]