电针介入对痛经模型大鼠子宫微循环的影响  

Effect of Electroacupuncture on the Uterine Microcirculation in Dysmenorrhea Rats

作　　者：李春华[1] 赵雅芳[1] 嵇波[1] 任晓暄[1] 郭孟玮[1] 丁喜艳[1] 孙琳琳[1] 张露芬[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《针刺研究》2011年第1期12-17,共6页Acupuncture Research

基　　金：国家重点基础研究发展计划课题(2006CB504503);国家中医药管理局针灸特色疗法评价重点研究室及针灸生物学三级实验室

摘　　要：目的:观察电针即刻介入对痛经模型大鼠子宫微循环的影响,进一步探讨针刺缓解胞宫疼痛的作用机制。方法:将动情间期雌性大鼠随机分为盐水组、模型组、三阴交组、悬钟组、非穴组,每组6只。除盐水组外,其余各组大鼠均连续10 d给予皮下注射苯甲酸雌二醇,末次给药1 h后,腹腔注射缩宫素2 U/只,制备痛经大鼠模型。于第10日各治疗组给予即刻电针20 min。采用XW-B-3冷光源微循环显微检查仪观察各组大鼠子宫微循环不同时段的变化。结果:与盐水组比,模型组微血管、毛细血管(cap)条数减少(P<0.05,P<0.01),微血管、cap粗细不均,管径收缩(P<0.01),血流减慢或停滞(P<0.01);与模型组比,即刻电针"三阴交"穴20 min时,微血管、cap条数均明显增多(P<0.05),微血管、cap管径均明显扩张(P<0.05),血流状态的差异无统计学意义(P>0.05);即刻电针"悬钟"穴、非穴各时段各指标与模型组相比差异均无统计学意义(P>0.05);与非穴组比,电针"三阴交"20 min时,cap管径明显扩张(P<0.05)。结论:"三阴交"穴电针即刻介入可缓解子宫血管的痉挛状态,改善子宫微循环,缓解疼痛,且穴位之间存在差异性。Objective To observe the effect of electroacupuncture （EA） on uterine microcirculation in dysmenorrhea rats so as to investigate its mechanism underlying relieving primary dysmenorrheal （PD）. Methods Female SD rats undergoing diestrus were randomly divided into saline control, model, Sanyinjiao（SP 6）, Xuanzhong（GB 39） and non-acupoint groups, with 6 rats in each. PD model was established by subcutaneous injection of estradiol benzoate for 10 days and intra-peritoneal injection of oxytocin （2 U） 1 h after the last estradiol injection. EA （2 Hz/100 Hz, 1 - 1.5 mA） was applied to the above-mentioned acupoints and non-acupoint area for 20 minutes. Numbers and diameters of the uterine microvessels （11- 100 μm） and capillaries （≤10 μm）, and the state of the uterine microcirculation were observed by using a Cold Light Microcirculation Detector. Results in comparison with the saline control group, the numbers and diameters of uterine microvessels and capillaries at the corresponding time-points 5 min, 10 min and 20 rain were decreased significantly in the model group （ P〈0.05, P〈0.01 ）. While compared with the model group, the numbers and diameters of uterine microvessels and capillaries at 20 min after EA in the SP 6 group were increased significantly （ P〈0.05）. The diameter of uterus capillaries of SP 6 group was significantly bigger than that of the non- acupoint group at the time-point 20 min （ P〈0.05）. No significant differences were found among the SP 6, GB 39 and non-acupoint groups in the diameter of uterine microvessels （P〈0.05）. Conclusion EA of SP 6 can effectively increase the number of uterine microvessels and capillaries and the diameter of the uterine microvessels and capillaries in PD rats, which may contribute to its effect in relieving dysmenorrhea by improving uterine microcirculation.

关 键 词：痛经 子宫微循环 电针 三阴交 悬钟 

分 类 号：Q463[生物学—生理学]