反复电针对佐剂性关节炎大鼠伏膈核-尾状核区大麻素CB1受体与多巴胺D1受体基因表达的影响  

Effects of Repeated Electroacupuncture on Gene Expression of Cannabinoid Receptor-1 and Dopamine 1 Receptor in Nucleus Accumbens-Caudate Nucleus Region in Inflammatory-pain Rats

作　　者：寿崟[1] 赵颖倩[1] 徐鸣曙[2] 葛林宝 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海市针灸经络研究所脑神经生物实验室,上海200030 [3]上海市气功研究所,上海200030 [4]上海市针灸经络研究中心,上海201203

出　　处：《针刺研究》2011年第1期18-22,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划资助(No.2009CB522901);上海市科委科研计划项目课题基金资助(07DZ19722-5)

摘　　要：目的:研究在电针镇痛中大麻素CB1受体与多巴胺D1受体之间的相互关系。方法:将30只SD大鼠随机分为对照组、模型组、电针组、电针+拮抗剂组、激动剂组。对照组大鼠左侧踝关节腔内注射生理盐水(0.05 mL),其余各组左侧踝关节腔内注射完全弗氏佐剂(0.05 mL)造模。电针"足三里""昆仑"30 min,隔日治疗1次,共4次。检测各组大鼠缩爪反应潜伏期(PWL)的变化,并应用实时荧光定量PCR检测脑内伏膈核-尾状核区CB1受体和D1受体mRNA表达情况。结果:(1)造模后,模型组与对照组比较,PWL显著降低(P<0.01)。电针治疗后PWL延长,电针+拮抗剂组的镇痛效果显著弱于电针组(P<0.01);激动剂组与电针组镇痛效果差异无统计学意义(P>0.05)。(2)模型组与对照组比较,大鼠脑内伏膈核-尾状核区CB1受体mRNA表达水平无显著变化。电针+拮抗剂组较电针组和激动剂组在大鼠脑内伏膈核-尾状核区CB1受体mRNA表达水平显著降低。(3)相同部位的D1受体与CB1受体的mRNA表达变化趋势一致。结论:CB1受体对D1受体的作用可能参与了电针镇痛。Objective To observe the effect of repeated electroacupuncture （EA） on the expression of cannabinoid receptor-1 （OB 1） rr.RNA and dopamine 1 receptor （D 1） mRNA in Nucleus Accumbens （NAO）-Oaudate Nucleus （ON） region in inflammatory-pain rats, so as to study its underlying mechanism in analgesia. Methods A total of 30 SD rats were randomized into normal control, model, EA, EA＋ AM 251 and WIN 55212-2 groups, with 6 cases in each group. EA （2 Hz/100 Hz, 1 - 3 mA） was applied to ＂Zusanli＂（ST 36） and ＂Kunlun＇（BL 60） for 30 rain, once every other day, and 4 sessions all together. Arthritis model was established by injection of Freund＇s complete adjuvant 0.05 mL in the rat＇s left ankle. Thermal pain threshold （paw withdrawal latency, PWL） was detected before and after modeling and after repeated EA and/or intraperitoneal injection of AM 251 （an inverse antagonist at the CB 1 cannabinoid receptor, 0. 1 mg/100 g） and WIN 55212-2 （a potent cannabinoid receptor agonist, 0.2 mg/100 g）. The expression of OB 1 receptor mRNA and D 1 receptor mRNA in the NAC-ON region was measured by real time fluorescence quantitative-polymerase chain reaction. Results Compared with the control group, the pain threshold values of the model group was decreased significantly （P〈0.01）. In comparison with the model group, the pain threshold values of the EA group and WIN 55212-2 group were increased considerably on day 10 （P〈0.01）. No significant differences were found between the EA＋ AM 251 and model groups and between the EA and WIN 55212-2 groups in PWL after the treatment （P〈0.05）. Compared with the control group, both CB 1 R mRNA and D 1 R mRNA expression levels in the model group were increased slightly, while in comparison with the model group and EA＋ AM 251 group, CB 1 R mRNA and D 1 R mRNA expression levels in the EA group and WIN 55212-2 group were upregulated obviously, No significant differences were found between the EA ＋ AM 251 and model groups and betwe

关 键 词：电针 佐剂性关节炎 大麻素CB 1受体 多巴胺D1受体 伏膈核-尾核 

分 类 号：R245.97[医药卫生—针灸推拿学]