电针对氯胺酮成瘾大鼠海马CA1区酪氨酸羟化酶、c-fos表达的影响  

Effects of Electroacupuncture on Expression of Tyrosine Hydroxylase and c-fos in Hippocampal CA1 Area in Ketamine-addiction Rats

作　　者：王丽发[1] 徐臣利[1] 熊克仁[1] 

机构地区：[1]皖南医学院解剖学教研室,芜湖241002

出　　处：《针刺研究》2011年第1期23-27,共5页Acupuncture Research

基　　金：安徽省高等学校省级优秀青年人才基金项目(No.2009SQRZ182)

摘　　要：目的:探讨电针对氯胺酮滥用成瘾戒毒治疗的物质基础。方法:将40只SD大鼠随机分为正常对照组、生理盐水(10 mL/kg)对照组、氯胺酮(100 mg/kg)组、氯胺酮(100 mg/kg)加电针组。按上述设定剂量,经腹腔注射给药,每天1次,连续7 d。氯胺酮加电针组于给药1周开始低频(2 Hz)电针交替刺激双侧"足三里"与"三阴交"穴位,每次30 min,每天1次,连续1周。采用免疫组织化学染色方法显示大鼠海马CA1区酪氨酸羟化酶(TH)、c-fos的表达。结果:与正常对照组和生理盐水对照组比,氯胺酮组大鼠海马CA1区TH、c-fos免疫反应阳性神经元的数目和阳性产物的表达水平明显增强(P<0.05,P<0.01)。与氯胺酮组比,氯胺酮加电针组TH、c-fos免疫反应阳性神经元的数目和阳性产物的表达显著减弱(P<0.01)。结论:电针"足三里""三阴交"可明显抑制氯胺酮成瘾引起的海马CA1区TH、c-fos表达的增强,提示电针可能通过抑制海马多巴胺神经元的活动改善氯胺酮成瘾。Objective To explore the morphological basis of electroacupuncture （EA） in relieving addiction of keta- mine. Methods Forty Sprague-Dawley rats were randomized into normal control, normal saline （10 mL/kg）, model and keta- mine＋ EA groups. Ketamine-addiction model was established by intraperitoneal administration of ketamine （100 mg/kg）, once a day for 7 days. EA （2 Hz） was applied to ＂Zusanli＂ （ST36） and ＂Sanyinjiao＂（SP 6） for 30 min, once a day for 7 days. The expression of tyrosine hydroxylase （TH） and c-fos in the hippocampal CA 1 region was detected by immunohistochemistry. Results in comparison with the normal control group and normal saline group, the numbers of both TH immune-reaction （IR）-positive and c-fos IR-positive neurons in the hippocampal region in the model group were increased significantly （P〈0.05, P〈0.01 ）. Corre- spondingly, the expression of both TH IR-positive and c-fos IR-positive products in the hippocampal CA 1 region in the model group was upregulated evidently （P〈0.05, P〈0.01 ）. After EA of ＂Zusanii＂ （ST 36）-＂Sanyinjiao＂ （SP 6）, compared with the model group, the expression of both TH IR-positive and c-fos IR-positive products was downregulated considerably （P〈0.01）. Conclusion EA of ＂Zusanli＂（ST 36）-＂Sanyinjiao＂（SP 6） can downregulate ketamine-addiction induced increase of expression of tyrosine hydroxylase and c-fos in the hippocampal CA 1 region in ketamine-addiction rats, which may contribute to its effect in relieving ketamine addiction symptoms in clinic.

关 键 词：氯胺酮成瘾 酪氨酸羟化酶表达 C-FOS表达 电针 海马CA 1区 

分 类 号：R245.97[医药卫生—针灸推拿学]