“太冲”穴中等强度针刺对高血压大鼠血压及血浆内皮素含量的影响  

Effect of Moderate Acupuncture-stimulation of "Taichong" (LR3) on Blood Pressure and Plasma Endothelin-1 Levels in Spontaneous Hypertension Rats

作　　者：王家有[1] 唐纯志[2] 贺振泉[1] 张进[1] 郝木峰[1] 马春媚[1] 杨忠华[1] 赖新生[2] 

机构地区：[1]广州中医药大学基础医学院人体解剖学教研室,广州510006 [2]广州中医药大学针灸推拿学院,广州510405

出　　处：《针刺研究》2011年第1期36-39,共4页Acupuncture Research

基　　金：国家重点基础研究发展计划("973"计划)课题"穴位效应规律的研究"子课题(No.2005CB523308)

摘　　要：目的:以不同刺激参数的捻转手法针刺自发性高血压大鼠(SHR)"太冲"穴,比较其降压效应及对血浆内皮素-1(ET-1)的影响,探讨针刺"太冲"穴治疗原发性高血压的最佳降压手法参数,同时为针刺手法量学提供有益依据。方法:选用SHR28只,随机分为模型组、轻刺激组、中刺激组和重刺激组(每组均7只),并以同体重正常血压SD大鼠7只作为正常组。3个针刺组分别以不同参数针刺大鼠双侧"太冲"穴,每次针刺5 min,每天1次,在针刺结束后15 min采用BP-6无创血压测量系统对大鼠进行血压测定,针刺到第7天采用放射免疫法检测各组大鼠血浆ET-1含量。轻、中、重3种针刺参数捻转角度、频率以及是否穿透足底皮肤分别为:0°、0次/min、穿透足底皮肤,180°、60次/min、不穿透足底皮肤,360°、120次/min、穿透足底皮肤。结果:与轻刺激、重刺激组比较,针后第2天,中刺激组的收缩压显著降低(P<0.01);第6、7天,中刺激组收缩压显著低于模型组和轻、重刺激组(P<0.01)。组内比,中刺激组的收缩压于刺激后第2、5、6、7天明显降低(P<0.01)。模型组血浆ET-1水平显著高于正常组(P<0.01);中刺激组血浆ET-1水平显著低于模型组(P<0.01);而轻刺激和重刺激组的ET-1浓度与模型组比较,差异无统计学意义(P>0.05)。结论:"太冲"穴不同量化参数的针刺降压效应具有差异性,"太冲"穴中等强度刺激可降低高血压大鼠血压及血浆内皮素含量。Objective To observe the effect of different strength of acupuncture stimulation on blood pressure and plasma endothelin （E）-1 in spontaneous hypertension rats （SHR）, so as to seek a better acupuncture parameter for clinical treatment of hypertension. Methods Twenty-eight 9-week-old SHRs were randomized into mild-stimulation group, moderate-stimulation group, strong-stimulation group and model group（n = 7 in each group）. Seven normotensive SD rats served as a normal control group. Acupuncture stimulation with mild, moderate and strong stimulation was applied to bilateral ＂Taichong＂ （LR 3） for 5 rain, once daily for 7 days. Blood pressure （BP） was determined by using a non-invasive BP-6 detection system. Plasma ET-1 was assayed by radioimmunoassay. Results Compared with the model group, the systolic pressure of the moderate-stimulation group on the 6th and 7^th day was decreased significantly after acupuncture of ＂Taichong＂ （LR 3） （ P〈0.01 ）, being significantly lower than that of the mild- and strong-stimulation groups（P〈0.01 ）. In comparison with the normal control group, plasma ET-1 level in the model group was increased significantly （P〈0.01）, while compared with the model group, only that in the moderate-stimula- tion group was down-regulated considerably （P〈0.01）. No significant differences were found between the mild-stimulation and model groups, between the strong-stimulation and model groups, and between the mild-stimulation and strong-stimulation groups in plasma ET-1 level （P〈0.05）. Conclusion Moderate-stimulation of ＂Taichong＂ （LR 3） can lower blood pressure and plasma EA-1 level in spontaneous hypertension rats. The reduced level of plasma ET-1 may be one of its mechanisms underlying improving hypertension.

关 键 词：自发性高血压 针刺刺激强度 太冲穴 血浆内皮素 

分 类 号：R245.311[医药卫生—针灸推拿学]