NF-кB参与七氟烷预处理减轻大鼠心肌缺血再灌注损伤  

NF-кB participates the process of sevoflurane preconditioning on myocardium against ischemia-reperfusion injury in rats

作　　者：覃琴[1] 谢红[1] 王琛[1] 刘霞[1] 朱江[1] 吴雪梅[1] 

机构地区：[1]苏州大学附属第二医院麻醉科,江苏苏州215004

出　　处：《基础医学与临床》2011年第3期247-251,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30872453);江苏省自然科学基金(BK2008166);江苏省高校自然科学基础研究面上项目(08KJD32005);苏州市第十三批科技发展计划(社会发展及医药)(2008-11)

摘　　要：目的探讨七氟烷(SEVO)预处理中NF-кB P65的活性表达对大鼠在体心肌缺血再灌注损伤的保护作用。方法 SD大鼠随机分为13组(n=6):假手术(sham)组;单纯缺血再灌注(I/R)组;PTN组于心肌缺血前15 min腹腔内注射NF-кB P65特异性阻断剂小白菊内酯(PTN,500μg/kg);七氟烷缺血(SEVO-I/R)组吸入2.5%七氟烷30 min,继之15 min药物排出后行心肌缺血30 min再灌注2 h;PTN+SEVO-I/R组和SEVO-I/R+PTN组分别于七氟烷预处理前15 min和预处理后即刻腹腔内注射PTN,分别在心肌缺血前和缺血再灌注后即刻两个时间点取心肌标本。SEVO组吸入七氟烷30 min后停止吸入165 min取心肌标本。Western blot法测定NF-кB P65蛋白表达。结果心肌缺血前,SEVO-I/R组NF-кB P65蛋白表达较I/R组上调(P<0.05)。缺血再灌注后,I/R组NF-кB P65蛋白表达较sham组上调,而缺血再灌注后的SEVO-I/R组、sham组、SEVO组、PTN组、SEVO-I/R+PTN组和PTN+SEVO-I/R组较缺血前SEVO-I/R组NF-кB P65蛋白表达显著下调(P<0.05)。结论七氟烷预处理减轻缺血再灌注大鼠心肌损伤可能与预处理期间大量激活NF-кB P65相关。Objective To investigate the protective effects of NF-кB P65 active expression during sevoflurane preconditioning on rat myocardium against ischemia-reperfusion injury in vivo.Methods Seventy-eight male adult SD rats weighing 270～390 g were anesthetized with intraperitoneal pentobarbital sodium 50 mg/kg,tracheostomized and mechanically ventilated.PaCO_2 was maintained at 25～40 mmHg（1 mmHg=0.133 kPa）.I/R was produced by reversible occlusion of left anterior descending branch（LAD） of coronary artery for 30 min followed by 2 h reperfusion.Myocardium samples of all groups were collected before the time of myocardial ischemia and after the time of myocardial ischemia reperfusion respectively.In addition,（G）Sevoflurane group;Rats were received 2.5% sevoflurane for 30 min and then collected myocardium sample after 165 min.NF-κB P65 was determined by Western blot analysis.Results The expression of NF-κB P65 protein was significantly up-regulated in sevoflurane-I/Rgroup as compared to that of the sham group.The expression of NF-κB P65 protein was significantly up-regulated in simple-ischemic group than that in the sham group and the expression of NF-κB P65 protein was significantly down-regulated in sevoflurane-I/R group,sham group,SEVO group,PTN group,SEVO-I/R＋PTN group and PTN＋SEVO-I/R group as compared to that of simple-ischemic group.Conclusion Sevoflurane preconditioning has protective effects on ischemia-reperfusion myocardial injury in rats.This protective effect may be involved in the activation of NF-κB P65 during sevoflurane preconditioning.

关 键 词：核转录因子-КB 七氟烷 预处理 心肌缺血再灌注损伤 

分 类 号：R965[医药卫生—药理学]