应用微卫星DNA标记分析荷斯坦母牛系谱可靠性及影响因素  

Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers

作　　者：初芹[1,2] 张毅[1] 孙东晓[1] 俞英[1] 王雅春[1] 张沅[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《畜牧兽医学报》2011年第2期163-168,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2006BAD04A01;2006BAD04A13);公益性行业科研专项(nyhyzx07-036-13);国家奶牛产业技术体系资金;北京市科技计划(D08060500070801);863项目(2007AA10Z157);转基因专项(2009ZX08009-156B)

摘　　要：为了评估当前中国荷斯坦牛群体的系谱准确性,本研究选择15头种公牛及来自21个牛场的2 220头女儿牛,借助牛3号染色体(BTA3)上17个微卫星标记,对女儿-公牛标记基因型是否错配进行分析。结果,按照错配标记数大于等于3个为系谱错误的标准,本研究所涉及的母牛群中系谱错误率为16.62%。经对影响系谱错误的因素分析表明,场效应是主要的影响因素,不同场间系谱错误比例差异较大;由于公牛女儿在各牛场中分布不均匀,导致公牛效应也达到显著。研究结果提示在中国荷斯坦牛群体进行亲子鉴定是亟需和必要的。To verify the pedigree data of Chinese Holstein cows,17 microsatellites on BTA3 were genotyped in 15 Chinese Holstein sires and their 2 220 daughters,which came from 21 herds in Beijing.The paternity with conflicts between the putative sire and daughter in at least 3 markers was considered to be erroneous.The paternity error rate was 16.62% in the Chinese Holstein population.Factors affecting incorrect paternity were further analyzed and results showed that the paternity error rate varied extremely significantly among herds.Since the daughters of sires were uneven distributed in different farms,the effect of sire was also significant.These findings indicate that it＇s urgent to carry out paternity testing in the Chinese Holstein population.

关 键 词：微卫星 亲子鉴定 系谱错误 中国荷斯坦牛 

分 类 号：S813.1[农业科学—畜牧学]