一株H11N2亚型禽流感病毒的分离鉴定及遗传进化分析  

Isolation and Characterization of One H11N2 Subtype Avian Influenza Virus A/duck/Jiangxi/k0701/2009

作　　者：赵坤坤[1] 张妍[1] 段志强[1] 钟蕾[1] 朱艳梅[1] 赵国[1] 刘晓文[1] 刘文博[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《畜牧兽医学报》2011年第2期217-223,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：十一五国家科技支撑计划(2006BAD06A01);国家科技重大专项课题(2008ZX10004-013);国家蛋鸡产业技术体系(nycytx-41-G07)

摘　　要：为了解禽流感病毒在华东地区家禽体内的进化情况,作者对华东地区某活禽市场的家禽进行了禽流感病毒的流行病学监测,并分离鉴定出1株H11N2亚型禽流感病毒A/duck/Jiangxi/k0701/2009。对该株病毒全基因组的遗传进化分析表明A/duck/Jiangxi/k0701/2009的8个基因片段来源广泛,与亚洲及欧洲某些地区的毒株存在广泛的重组现象。其PB2基因和NS基因可能由A/mallard/Korea/GH170/2007(H7N7)提供,NA基因与高致病性禽流感病毒A/duck/Hebei/0710/2009(H5N2)的NA基因亲缘关系较近,而PB1、PA和NP基因则可能与A/Muscovy duck/Thailand/CU-LM1984/2009(H4N9)有共同来源。本研究结果提示该株病毒可能为1株多元重组病毒,且可能在高致病性禽流感病毒的进化中起了重要作用。To explore the evolutionary patterns of avian influenza virus（AIV） in poultry in Eastern China,epidemiological surveillance of AIVs was carried out in Eastern China,and one H11N2 subtype avian influenza virus,A/duck/Jiangxi/k0701/2009,was isolated from domestic duck in a live bird market.The analysis of complete genome sequences and genetic evolution showed that all eight segments of A/duck/Jiangxi/k0701/2009（H11N2） belong to Eurasian lineage,but genetic recombination was observed between A/duck/Jiangxi/k0701/2009（H11N2） and AIVs in some regions in Asia and Europe in varying degrees and extensively.Moreover,the PB2 and NS gene may be originated from A/mallard/Korea/GH170/2007（H7N7）,NA gene may have the same origin with NA of HPAIV A/duck/Hebei/0710/2009（H5N2）,and NP,PA,PB1 genes may have the same origin with A/Muscovy duck/Thailand/CU-LM1984/2009（H4N9）.Our results indicated that A/duck/Jiangxi/k0701/2009 may be a multi-origin reassortant virus,and may play an important role in the evolution of HPAIVs.

关 键 词：禽流感病毒 H11N2亚型 遗传进化分析 基因重组 

分 类 号：S852.659.5[农业科学—基础兽医学]