皖南黄肉种鸡种蛋中禽白血病病毒的感染状态检测  

Evaluation on ALV Infection in Fertilized Eggs from A Wan-nan Yellow-feather Parent Broiler Breeder Flock

作　　者：王波[1] 李清源[1] 刘绍琼[1] 张永光[2] 崔治中[1,3] 孙淑红[1,3] 

机构地区：[1]山东农业大学动物医学院,泰安271018 [2]中国农业科学院兰州兽医研究所,兰州730046 [3]山东省动物生物技术与疫病防治重点实验室,泰安271018

出　　处：《畜牧兽医学报》2011年第2期224-227,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：家畜疫病病原体生物学国家重点实验室开放课题资助(SKLVEB2010KFKT001);国家公益性行业(农业)科技专项(200803019)

摘　　要：本研究首次通过检测种蛋中禽白血病病毒（ALV）评价了皖南黄父母代肉种鸡的ALV感染状态。收集该鸡群的120枚鸡蛋,取40枚鸡蛋孵化至9~11 d后分别制备CEF,培养10 d,取细胞上清用ELISA试剂盒检测ALV p27抗原。另取80枚鸡蛋卵白直接检测p27抗原,同时分别将80枚鸡蛋卵白接种CEF（DF1）,培养10 d后取细胞上清检测p27抗原。比较p27阳性检出率的结果表明,受精蛋孵化9~11 d后制备CEF,再培养10 d的细胞上清检出率（10/36）高于直接检测卵白（17/80）与卵白接种CEF（DF1）的细胞上清（7/80）。将经DF1培养后p27抗原阳性样品,用针对ALV-J的单抗JE9做IFA,ALV-J的阳性率为6/7。检测该80枚鸡蛋的卵黄抗体,ALV-J、ALV-AB的抗体阳性率分别为18/80、1/80。结果表明,该皖南黄父母代肉种鸡群存在的外源性ALV感染主要为ALV-J。该研究为ALV的净化提供了可行性检测方法。In the present study,the infection of avian leukosis virus（ALV） in parent breeder flock was evaluated by the inspection of fertilized eggs.120 eggs were obtained for a flock of Wan-nan yellow-feather parent layers.40 eggs were hatching for 9 to 11 days for the preparation of CEF,which were cultured for 10 days and the supernatants were collected for the measurement of p27 antigen by ELISA.The egg white was obtained from the other 80 eggs and used for the direct measurement of p27 antigen or indirect measurement after inoculation with CEF（DF1） and cultured for 10 days.The results showed that ALV positive rate（10/36） was higher in the measurement with cultured CEF than that in the determination with egg white（17/80） or egg white inoculated with CEF（7/80）.The ALV-J positive rate in DF1 positive samples was 6/7 by the measurement of IFA with JE9,a monoclonal antibody to ALV-J.The positive rates of ALV-J and ALV-AB in egg yolk antibody were 18/80 and 1/80,respectively.The results showed that the parent flock was infected with ALV-J.The result suggests that the measurement of ALV antigen from eggs is an available method for the detection of ALV infection.

关 键 词：皖南黄肉种鸡 种蛋 禽白血病病毒 感染状态 

分 类 号：S852.659.3[农业科学—基础兽医学]