日粮中玉米赤霉烯酮和大豆异黄酮联合作用对后备母猪生殖器官发育和雌激素受体基因转录的影响  

Interactive Effects of Dietary Zearalenone and Soybean Isoflavone on Development of Reproductive Organs and Transcription of Estrogen Receptors Gene in Replacement Gilts

作　　者：王定发[1] 彭运智[1] 张妮娅[1] 齐德生[1] 

机构地区：[1]华中农业大学动物科技学院,武汉430070

出　　处：《畜牧兽医学报》2011年第2期243-250,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30771561/C120104)

摘　　要：本试验旨在研究玉米赤霉烯酮(Zearalenone,ZEA)和大豆异黄酮(Soybean isoflavone,ISO)二者联合作用对后备母猪生长性能、生殖器官发育及生殖器官雌激素受体(Estrogen receptors,ERs)基因转录的影响。采用3×3两因子析因法设计,将90头75日龄的D×L×Y三元杂交后备母猪随机分为9个处理,每个处理5个重复,每个重复2头猪。各处理组在基础日粮基础上分别添加ZEA 0、0.5、2 mg.kg-1和ISO 0、300、600 mg.kg-1。试验时间为21 d。结果表明:日粮中同时添加ZEA和ISO对后备母猪平均日增体质量(ADG)和平均日采食量(ADFI)影响不显著(P>0.05)。日粮中同时添加ISO和0.5 mg.kg-1ZEA与单独添加0.5 mg.kg-1ZEA处理组相比,生殖器官质量和阴户宽度增加。但日粮中添加ISO可缓解2 mg.kg-1ZEA导致的生殖器官增加质量和阴户红肿(P<0.05),降低子宫和阴道组织ERαmRNA的转录水平,而增加了ERβmRNA转录水平。结果提示,日粮中添加ZEA和ISO联合作用对后备母猪ADG和ADFI无显著影响,日粮中ISO和低剂量的ZEA联合作用使雌激素作用增强,但可缓解高剂量ZEA的雌激素效应,这种联合作用对生殖器官发育的影响是通过ISO的双相调节作用和改变生殖器官ERs的mRNA转录来调控的。To study the interactive effects of zearalenone（ZEA） and soybean isoflavone（ISO） on growth performance,development of reproductive organs and transcription of estrogen receptor genes in replacement gilts,ninety 75-day-old female crossed-bred pigs（Duroc × Landrace × Yorkshire） were randomly allocated to nine diet treatments with 5 replicates in each treatment of 2 pigs per replicate during the 21-day research.The experiment was a 3×3 factorial design using a none-soybean meal diets with the level of ZEA at 0,0.5 or 2.0 mg·kg-1 and the addition of ISO at 0,300 or 600 mg·kg-1.The results showed that interaction of ZEA and ISO in diets has no significant influences on the average daily gain（ADG） and average daily feed intake（ADFI） in the replacement gilts（P0.05）.ISO could enhance the increased weight of the reproductive organs and the width of vulva induced by ZEA with 0.5 mg·kg-1 in diets.However,ISO with 300-600 mg·kg-1 in diets could reduce the increased weight of reproductive organs and edema of vulva induced by ZEA with 2 mg·kg-1 in diets of replacement gilts（P0.05）.Simultaneous provision of ISO and 2 mg·kg-1 ZEA to replacement gilts increased the mRNA transcription of ERα,and reduced the mRNA transcription of ERβ in uterus and vagina compared with the pigs offered the diets containing 2 mg·kg-1 ZEA alone.The results suggested interaction of ZEA and ISO in diets has no significant influences on ADG and ADFI in the replacement gilts.The estrogenic effect of ZEA would be reinforced when coexistence of ISO and low dosage of ZEA（0.5 mg·kg-1） in the diets.While ISO could offset the estrogenic effect of ZEA when the diets containing the high dosage of ZEA（2 mg·kg-1）.The effects of ZEA and ISO on the development of reproductive organs in replacement gilts were mediated through the dual estrogen function of ISO and changing the mRNA transcription of ERs in the reproductive organs.

关 键 词：玉米赤霉烯酮 大豆异黄酮 后备母猪 生殖器官 雌激素受体 联合作用 

分 类 号：S852.2[农业科学—基础兽医学] S828[农业科学—兽医学]