力竭性运动后大鼠血清CK、CK-MB活性和心肌组织形态学的动态改变  

The dynamic change of serum CK,CK-MB and myocardium histomorphology after exhausted exercise in rats

作　　者：王福文[1] 赵敬国[2] 王燕[1] 李杰[1] 胡志力[1] 

机构地区：[1]山东省医学科学院药物研究所,山东省现代医用药物与技术重点实验室,济南250062 [2]山东师范大学体育学院,济南250014

出　　处：《中国应用生理学杂志》2011年第1期52-55,134,共5页Chinese Journal of Applied Physiology

基　　金：山东省自然科学基金项目(ZR2009CM089);山东省教育厅科技计划项目(J07YE09);山东省医学科学院资助课题(07035)

摘　　要：目的：探讨一次和反复力竭性运动后不同时相大鼠血清肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）与心肌损伤的变化规律。方法：通过力竭性游泳制备运动性心肌损伤模型,分别于运动后即刻和3 h6、h、12 h2、4 h4、8 h、96 h检测血清CK、CK-MB活性,并观察心肌组织形态学的动态变化。结果：大鼠一次力竭运动后0~12 h,CK和CK-MB活性明显增加,6 h达高峰;心肌炎细胞浸润灶逐渐增多,胞质嗜酸性增强,损伤高峰在12 h左右。反复力竭运动后0~12 h和48 h9、6 h CK和CK-MB活性皆明显增加,分别于运动后即刻和96 h达高峰;心肌细胞均有不同程度的损伤,48 h最严重。结论：过度运动和/或力竭性运动皆引起运动性心肌损伤,同时存在延迟性心肌损伤。Objective： To study the dynamic changes of serum CK,CK-MB and myocardium histomorphology in different time periods after single bout and repeated exhausted exercise in rats.Methods： The animal models of myocardial injury were established by exhausted swimming.Creatine kinase（CK）,creatine kinase mass（CK-MB） activities in serum were measured immediately at 3,6,12,24,48 and 96 hours after exhausted exercise,and the dynamic changes of myocardial histopathology were examined.Results： The CK,CK-MB activities were significantly increased immediately at 3,6,12 hours and peaked at 6 hours after single bout of exhausted exercise,meantime the degree of inflammatory cell infiltrate and strong acidophil staining were gradually increased in myocardium of rat,and the myocardial injury was most severe at 12 hours.After 1-week consecutive daily exhausted swimming,CK,CK-MB in serum were obviously increased immediately at,3,6,12,48 and 96 hours postexercise and peaked immediately and at 96 hours respectively postexercise.There were different degrees of myocardial injury in different time of recovery phase,and was most severe at 48 hours postexercise.Conclusion： The myocardial injury was induced by excessive exercise and/or exhausted exercise,and the resulting delayed-onset myocardial injury was further certified.

关 键 词：力竭运动 心肌损伤 CK-MB 病理组织学 

分 类 号：G804.23[文化科学—运动人体科学]