全反式维甲酸抑制人肺腺癌A549细胞迁移的机制探讨  

Mechanism of all-trans-retinoic acid in inhibiting the migration of human lung adenocarcinoma A549 cells

作　　者：桂淑玉[1,2] 王银凤[1] 程英[1] 周青[3] 陈飞虎[4] 汪渊[3] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,安徽合肥230022 [2]安徽医科大学分子生物学实验室,生物化学教研室和安徽省重要遗传病基因资源利用重点实验室,安徽合肥230022 [3]安徽医科大学分子生物学实验室生物化学教研室和安徽省重要遗传病基因资源利用重点实验室,安徽合肥230022 [4]安徽医科大学药学院,安徽合肥230022

出　　处：《中国药理学通报》2011年第1期33-36,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30971226);安徽省自然科学基金资助项目(No090413116)

摘　　要：目的探讨全反式维甲酸(ATRA)对人肺腺癌细胞迁移的影响及其作用机制。方法细胞划痕实验检测ATRA对A549细胞迁移的影响;Western blot分析肌球蛋白轻链激酶(MLCK)的表达和肌球蛋白轻链(MLC)磷酸化程度;观察MLCK抑制剂ML-7是否影响A549细胞的迁移能力。结果 1 mg.L-1 ATRA处理的A549细胞和细胞对照以及溶剂对照细胞相比,细胞的迁移距离无明显变化,而10 mg.L-1ATRA可明显降低细胞的迁移距离(P<0.05)。Western blot分析结果表明10 mg.L-1 ATRA可明显降低A549细胞ML-CK的表达和MLC磷酸化(P<0.05);ML-7可明显降低A549细胞迁移(P<0.05)。结论 ATRA通过降低MLCK的表达和MLC磷酸化,抑制A549细胞的迁移。Aim To explore the influences of all-trans-retinoic acid（ATRA） on the migration of human lung adenocarcinoma cells and its mechanism.Methods The impact of ATRA on the migration of A549 cells was measured by scarification test.The effect of ATRA on the expression of myosin light-chain kinase（MLCK） and the phosphorylation of myosin light chains（MLC） in A549 cells were analyzed by Western blot,and the influence of ML-7,a selective inhibitor of MLCK,on the migration of A549 cells was observed.Results Compared with cell and solvent control group,the migration distance of A549 cells treated with 10 mg·L-1 ATRA for 24 h was obviously decreased（P0.05）.The Western blot analysis showed that 10 mg·L-1 ATRA could significantly decrease the expression of MLCK and the phosphorylation of MLC in A549 cells（P0.05）.The decurtation of migration distance of A549 cells was achieved by ML-7.Conclusions ATRA may inhibit the migration of A549 cells through decreasing the expression of MLCK and the phosphorylation of MLC.

关 键 词：全反式维甲酸 人肺腺癌A549细胞 迁移 肌球蛋白轻链激酶 表达 细胞划痕实验 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R345.57[医药卫生—基础医学]