2-甲氧基雌二醇对CEM细胞增殖的影响及其机制研究  

Effects of 2-methoxyestradiol on CEM cells and its underlying mechanisms

作　　者：张学亚[1] 潘敬新[1] 郭熙哲[1] 战榕[2] 

机构地区：[1]福建医科大学附属第二医院血液科,福建泉州362000 [2]福建医科大学附属协和医院血液科,福建福州350001

出　　处：《中国药理学通报》2011年第1期103-106,共4页Chinese Pharmacological Bulletin

基　　金：福建省卫生厅青年课题基金资助(No2006-1-38);福建省科技厅资助省属高校项目(No2006F5041)

摘　　要：目的研究2-甲氧基雌二醇(2-ME2)对人白血病细胞系CEM细胞的作用及其分子机制。方法采用MTT法检测不同浓度2-ME2处理CEM细胞48 h后细胞的增殖活性;2μmol.L-1 2-ME2处理CEM细胞0、24、48和72 h,采用RT-PCR法检测VEGF和hTERT mRNA的表达情况,Westernblot法检测Akt和p-Akt蛋白表达变化。结果不同浓度2-ME2能够有效抑制CEM细胞增殖,并呈量效关系,2μmol.L-1 2-ME2处理CEM细胞24、48和72 h可降低CEM细胞中的VEGF和hTERT mRNA表达,并降低Akt蛋白磷酸化水平,而总Akt蛋白表达无变化。结论 2-ME2能够有效抑制CEM细胞增殖,其可能作用机制与下调hTERT和部分通过下调VEGF mRNA表达,阻断PI3K/Akt信号通路转导有关。Aim To study the effect of 2-methoxyestradiol（2-ME2）on human leukemia CEM cells and its underlying mechanisms.Methods CEM cells were treated with different concentrations of 2-ME2 at 48 h.MTT assay was used to examine the effect of growth inhibition.CEM cells was treated with 2 μmol·L-1 2-ME2 at 0,24,48 and 72 h.The expression of VEGF and hTERT mRNA were detected by RT-PCR;the expression of Akt and p-Akt proteins were detected by Western blot.Results Different concentrations of 2-ME2 inhibited proliferation of CEM cells in a dose-dependent manner with the IC50 was 2 μmol·L-1;2-ME2 decreased the expression of VEGF and hTERT mRNA,and down-regulated proteins level of p-Akt,while it had no effect of total Akt protein in CEM cells after treated with 2 μmol·L-1 of 2-ME2 for 0,24,48 and 72 h.Conclusions 2-ME2 decreases the expression of hTERT and interferes in PI3K/Akt signaling pathway through partly decreasing the expression of VEGF mRNA,which provides a possible mechanism for explaining the 2-ME2＇s antiproliferative activity.

关 键 词：2-甲氧基雌二醇 白血病 CEM细胞 AKT HTERT VEGF 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R341[医药卫生—基础医学]