口腔癌相关成纤维细胞的特殊体外行为研究  

Special biological behavior in vitro of oral carcinoma associated fibroblasts

作　　者：张红闯[1] 万延俊[1] 李家锋[1] 贺文鹏[1] 张扬[1] 梁军铭[1] 

机构地区：[1]徐州医学院附属徐州市立医院口腔科,徐州221002

出　　处：《口腔医学》2011年第2期84-86,共3页Stomatology

摘　　要：目的探讨口腔颊癌中癌相关成纤维细胞(Carcinoma Associated Fibroblasts,CAFs)的特殊体外生物学行为。方法组织块贴壁法培养CAFs及正常颊黏膜成纤维细胞(Normal Fibrolasts,NFs);通过体外生长形态观察,增殖、粘附行为差异的区别,比较两种细胞的生物学行为差别。结果与NFs相比,CAFs均具有更高的增殖活性、贴壁率及迁移能力(P<0.05)。结论口腔癌CAFs的体外增殖、粘附能力均高于NFs,这或许提示其在口腔癌的侵袭、转移中发挥了重要的作用。Objective To investigate the biological behavior in vitro of carcinoma associated fibroblasts（CAFs）.Methods Primary cultured CAFs and normal fibroblasts（NFs）of buccal in vitro were prepared.Related research was conducted on the biological disparity between the two strains in morphology,proliferative activity and adhesion behavior.Results Evident disparity of proliferative activity,adherence and migration was discovered between CAFs and NFs（P0.05）.Conclusions The ability of generation,migaration and adhesion of CAFs exceeded NFs in vitro.This may suggest CAFs have played an important role in oral cancer invasion and metastasis

关 键 词：口腔鳞癌 癌相关成纤维细胞 体外培养 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]