G蛋白抑制肽GCIP-27对大鼠慢性心力衰竭的影响  

Effects of G protein inhibitory peptide GCIP-27 on chronic heart failure in rats

作　　者：卢小岚[1] 杨华 徐亚莉[2] 刘雅[1] 王妍 李晓辉[1] 张海港[1] 

机构地区：[1]中国人民解放军第三军医大学药学院药剂学教研室暨新药研究中心,重庆400038 [2]中国人民解放军第三军医大学第二附属医院超声科,重庆400037

出　　处：《中国新药与临床杂志》2011年第2期126-130,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-052);国家自然科学基金(30973524);重庆市科技攻关项目(CSTC2009AB5034)

摘　　要：目的探讨G蛋白抑制肽GCIP-27对多柔比星(Dox)诱导的大鼠慢性心力衰竭的作用。方法 48只SD大鼠随机分为对照组、模型组、氯沙坦组、GCIP-27组,分别给予生理盐水、Dox、Dox+氯沙坦、Dox+GCIP-27(10、30、90μg·kg^(-1),ip,bid)。给予Dox复制模型后,再继续喂养大鼠,于d71,经胸高频彩超诊断仪测定大鼠心功能;称量大鼠体重、心脏质量、左心室质量,计算心脏质量指数和左心室质量指数;HE染色后观察大鼠心肌组织病理形态学改变。结果 Dox可明显诱导大鼠发生慢性心力衰竭;氯沙坦6mg·kg^(-1)、GCIP-27(30、90μg·kg^(-1),bid)能明显抑制大鼠左心室重构,提高心脏的射血分数和短轴缩短率,降低心脏质量指数和左心室质量指数,减少心肌组织的损伤。结论 GCIP-27能抑制慢性心力衰竭大鼠的左心室重构,改善心脏功能。AIM To explore the effects of G protein inhibitory peptide GCIP-27 on doxorubicin-induced chronic heart failure in rats. METHODS Forty-eight rats were randomly divided into six groups. The rats in control, doxorubicin, losartan and GCIP-27 groups were respectively administrated with normal saline, doxorubicin, losartan 6 mg·kg^-1 and GCIP-27 10, 30, 90 μg·kg^-1 （ip, bid） . After 10 weeks treatment, the heart function of all the rats were determined by high frequency echocardiography, and then all the rats were killed and cardiac mass index and left ventricular mass index were measured. The pathological change was analyzed by histological hematoxylin-eosin staining. RESULTS Losartan 6 mg· kg^-1 and GCIP-27 （30, 90 μg·kg^-1） markedly inhibited left ventricular remodeling, improved cardiac ejection fraction and shortened fraction, decreased cardiac mass index and left ventricular mass index, and reduced the myocardial tissue injury. CONCLUSION GCIP-27 can improve heart function effectively in rats with chronic heart failure induced by doxorubicin.

关 键 词：肽类 GTP结合蛋白质类 心力衰竭 多柔比星 GCIP-27 

分 类 号：R965[医药卫生—药理学]