原发性肾病综合征患儿外周血单个核细胞膜P-糖蛋白170表达的意义  

Significance of Expression of PGlycoprotein 170 in Peripheral Blood Mononuclear Cells of Children with Primary Nephrotic Syndrome

作　　者：关凤军[1,2] 易著文[1] 党西强[1] 何庆南[1] 吴小川[1] 何小解[1] 黄丹琳[1] 

机构地区：[1]中南大学湘雅二医院儿科肾病研究室,长沙410011 [2]徐州医学院附属医院儿科

出　　处：《实用儿科临床杂志》2011年第5期322-324,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨P-糖蛋白(P-gp)170在肾病综合征(NS)患儿糖皮质激素(GC)耐药中的作用及其介导耐药的可能机制。方法选取24例初发未应用GC的NS患儿为病例组,根据随访结果将其分成GC敏感型NS组(SSNS组)和GC耐药型NS组(SRNS组)。10名健康体检儿童为健康对照组。将健康对照组和病例组的外周血单个核细胞(PBMC)均予地塞米松(Dex)处理,培养后分别在0 h、12 h、24 h、36 h不同时间点进行检测,同时以不加Dex作为空白对照,Western blot法检测SSNS组和SRNS组患儿PBMC膜P-gp170的表达量,并结合临床资料进行比较。结果 1.SSNS组空白对照各时间点PBMC膜表面P-gp170表达量比较差异无统计学意义(P>0.05),与健康对照组空白对照比较,该组各时间点P-gp170的表达量较高(P<0.05);Dex处理SSNS组患儿PBMC膜表面P-gp170表达随培养时间延长而渐降低(P<0.05),与Dex处理健康对照组比较,各时间点P-gp170的表达量较高(P<0.05);与SSNS组患儿空白对照比较,Dex处理SSNS组患儿PBMC膜表面各时间点P-gp170表达量较高(P<0.05)。2.SRNS组患儿空白对照PBMC膜表面P-gp170表达随培养时间不同而无明显变化,不同时间点比较差异无统计学意义(P>0.05),与健康对照组空白对照比较,该组表达量升高(P<0.05),Dex处理SRNS组患儿PBMC膜表面P-gp170表达随培养时间的不同而无变化,各时间点P-gp170的表达量差异无统计学意义(P>0.05);与SRNS组患儿比较,SSNS组患儿空白对照各时间点P-gp170表达均较低(Pa<0.05),Dex处理SSNS组患儿各时间点P-gp170表达均较低(Pa<0.05);与SRNS组患儿空白对照比较,Dex处理SRNS组患儿各时间点PBMC膜表面P-gp170表达量比较差异无统计学意义(P>0.05)。结论 1.健康儿童和NS患儿PBMC均有P-gp170表达,NS状态下可有P-gp170表达增高。2.SRNS患儿PBMC膜P-gp170表达增高可能与GC耐药有关,P-gp170表达增高可能介导了部分NS患儿GC耐药。3.NS患儿PBMC膜P-gp170表达增高与GC应用有关,GC可能Objective To explore the role of P-glycoprotein（P-gp）170 in steroid resistant nephrotic syndrome（NS） and the mechanism involved. Methods Twenty-four children with NS without any glucocorticoid（GC） treatment before GC administration were divided into 2 groups：steroid-sensitive NS（SSNS） group and steroid-responsive NS（SRNS） group according to the results of follow-up,with 10 healthy children serving as healthy control group.Each subject was treated with dexamethasone（Dex） and cultivated,and Western blot assay was adopted to test P-gp170 expression in membrane of peripheral blood mononuclear cells（PBMC） at different time points,namely at 0 h,12 h,24 h and 36 h,and at the same time,samples without Dex treatment serving as blank control. Results 1.No change was found of P-gp170 expressions in SSNS blank at different time points,thus no significant difference was found among them according to statistics（P0.05）;P-gp170 expressions in SSNS blank control at different time points were all higher compared with healthy blank control group,thus significant differences were found among them according to statistics（P0.05）;P-gp170 expression in Dex-treated SSNS decreased by and by,with a significant difference among different time points statistically（P0.05）;P-gp170 expression at different time points were all higher in Dex-treated SSNS compared with Dex-treated healthy controls,thus a significant difference was found among them according to statistics（P0.05）;P-gp170 expression in Dex-treated SSNS were all higher than those of SSNS blank controls,thus a significant diffe-rence was found at each time point statistically（P0.05）.2.No change was found of P-gp170 expression in SRNS at different time points,thus no significant difference was found among them according to statistics（P0.05）;and there was difference of P-gp170 expression compared with healthy blank controls statistically（P0.05）;No change was found of P-gp170 expression in Dex-treated SRNS at different times,thus n

关 键 词：P-糖蛋白170 肾病综合征 糖皮质激素耐药 儿童 

分 类 号：R726.9[医药卫生—儿科]