冠心病心血瘀阻证和心肾阴虚证的蛋白组学研究  

Study on the Syndrome of Stagnant Blockade of Heart Blood and yin Deficiency of Heart and Kidney of Coronary Heart Disease Through Proteomics

作　　者：王刚[1] 姜民[1] 朱明丹[2] 崔佩佩[1] 杜武勋[2] 范应昌[3] 罗国安[1,4] 

机构地区：[1]南开大学药学院,天津300071 [2]天津中医药大学第二附属医院,天津300150 [3]天津中医药大学,天津300193 [4]清华大学生命科学与医学研究院,北京100084

出　　处：《辽宁中医杂志》2011年第3期405-407,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(90709003)

摘　　要：目的:使用蛋白质组学技术对冠心病心血瘀阻证和心肾阴虚证这两种证候之间差异进行研究,以期找到两种证候间差异的物质基础并初步阐释两者差异的信号通路。方法:采集冠心病心血瘀阻证和心肾阴虚证病人的血浆,用荧光差异蛋白电泳(2D-DIGE)、质谱(MALDI-TOF)等蛋白质组学技术分析和鉴定两种证候间蛋白表达的变化,并用生物信息学方法对其涉及的主要信号通路进行分析。结果:蛋白组学研究发现冠心病心血瘀阻证和心肾阴虚证相比共有10种差异表达蛋白,其中7种表达上调,3种表达下调。涉及的通路主要有补体和凝血级联通路及阿尔兹海默症信号通路。结论:补体和凝血级联通路及阿尔兹海默症信号通路与冠心病心血瘀阻证和心肾阴虚证的中医分型有关,但需要进一步验证和机制研究。Objective ： To indentify specific protein markers for the syndrome of stagnant blockade of heart blood and yin deficiency of heart and kidney of coronary heart disease and interpret the pathway of the difference. Methods： The blood plasma samples of CHD patient with the syndrome of stagnant blockade of heart blood and yin deficiency of heart and kidney were collected to identified the protein expressed differential between the two syndrome by proteomic techniques such as fluorescence differential ingel electrophoresis（ 2D- DIGE ）and MALDI-TOF, and then analysis major signaling pathway involved with Biolnformatics approach. Results：lO proteins are detected differential between the patient with the two syndrome,7 of all are up regulated and 3 are down regulated. The primary pathways involved includ complement and coagulation cascades pathway and alzheimers disease pathway. Conclusion：It is proved that complement and coagulation cascades pathway and alzheimers disease pathway are related to differentiation for syndrome classification of syndrome of stagnant blockade of heart blood and yin deficiency of heart and kidney. But the conclusion needs further validation and mechanism studies.

关 键 词：冠心病 心血瘀阻证 心肾阴虚证 蛋白组学 

分 类 号：R541.4[医药卫生—心血管疾病]