HPLC法测定化痔栓中人参皂苷Rg_1、三七皂苷R_1的含量  

Determination of Ginsenoside Rg_1,Notoginsenoside R_1 in Hua Zhi Suppository by HPLC

作　　者：王桂香[1] 范晓文[2] 张春玲[3] 孙爽[2] 

机构地区：[1]鞍山师范学院附属卫生学校,辽宁鞍山114001 [2]沈阳药科大学,辽宁沈阳110016 [3]沈阳红药制药有限公司,辽宁沈阳110044

出　　处：《辽宁中医杂志》2011年第3期519-520,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:研究化痔栓中人参皂苷Rg1、三七皂苷R1的含量测定方法。方法:采用高效液相色谱法进行梯度洗脱,色谱柱为C18柱(4.6mm×250mm,5μm),流动相为乙腈∶水(19∶81),乙腈∶水(36∶64),流速为1.0mL/min,检测波长为203nm,柱温为室温。结果:人参皂苷Rg1、三七皂苷R1的线性良好(r=0.9998、r=0.9997),线性范围人参皂苷Rg197.6～976.0μg/mL,三七皂苷R122.0～220.0μg/mL。人参皂苷Rg1、三七皂苷R1的回收率分别为97.82%、97.60%;RSD值分别为1.26%、1.84%,n=5。结论:该方法简便,灵敏,重现性好,可作为化痔栓中人参皂苷Rg1、三七皂苷R1的含量测定方法。Objective：To study a method of determing ginsenoside Rg,, notoginsenoside R1 contents in Huao Zhi suppository. Methods：Chromatographlc conditions are as follows ：C18 column （4.6mm×250mm,5μm） as the stationary phase, acetonltrile ：water（19：81） ,acetonitrile：water（36： 64） as the mobile phase,the velocity of flow is 1.0mL/mln,the temperature of coloum is room temperature and the detection wavelength is 203 nm. Results：The HPLC method has good lincarity in determining the total saponin of Panax notoginseng content, and we can detect two saponins at the same time, linera range, ginsenoside Rg1 97.6 - 976.0μg/mL and notoginsenoside R1 22.0 -220.0μg/mL, the correlation coefficient r = 0. 9998 and r = 0. 9997. The average recoveries were 97.82% for ginsenoside Rg, （ RSD = 1.26% ） ,97.60% for notoginsenoside R, （ RSD = 1.84% ）, n = 5, respec - tively. Conclusion ： The method is simple, sensitive and precise. It could be used for determination of ginsenoside RgI and notogin- senoside R1 contents in Hua Zhi suppository.

关 键 词：人参皂苷RG1 三七皂苷R1 HPLC 

分 类 号：R284.1[医药卫生—中药学]