光学相干成像结合反射光谱技术对小鼠烫伤模型的评估  

Mice Scald Model Investigated by OCT Combined with Reflection Spectrum

作　　者：张浩[1,2] 孟耀勇[1] 张小燕[1] 肖军[1] 刘颂豪[1] 

机构地区：[1]华南师范大学光子中医实验室,广东广州510631 [2]中山大学物理科学与技术学院,广东广州510275

出　　处：《光谱学与光谱分析》2011年第4期960-965,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60411130595);广东中医药局科研项目(2008233)资助

摘　　要：光学相干层析成像（OCT）和反射光谱技术用于精确地评估小鼠烫伤模型。在相同作用时间不同温度下,皮肤厚度呈线性增加;皮下肌肉组织拟合吸收系数和斜率呈V字型变化,在80℃时,处在最低处;在65~85℃时,光谱一次微商的波峰与波谷明显分离,85℃之后这种能力逐渐减弱;色坐标值中,红色数值在60~70℃和90~95℃两个温度之间急剧减小,代表绿色数值一直缓慢减小,而蓝色数值增加。相同温度不同热作用时间下,皮肤的厚度略微减小;拟合吸收系数在热作用少于20 s时间内数值较大,在30 s后数值较小;构造的斜率变化很小;色坐标值在作用时间超过20 s时,变得相对稳定。以上参数主要发生在作用时间为10 s,超过这个时间,变化相对很小。这些变化能够反映出皮肤和皮下组织在热作用下,充血带、水肿瘀滞带和凝固带的形成和转化。这两种技术结合能够为临床提供一种实时的、低消费的、非侵入的在体的烫伤检测的新的光学活检手段。OCT combined with reflective spectrum was employed to precisely evaluate the mice scald model.Under the conditions that the temperature increased by 5 ℃ from 60 to 95 ℃ with 20 s thermal effect,the cutis depth linearly increased.The fitting absorption parameters and the gradients showed the V trends.Wave peaks of first order differential were legible from 65 to 85 ℃,then wave peaks became worse over 85 ℃.At color coordinates,red value decreased severely from 65 to 85 ℃ and from 90 to 95 ℃,green value continuously decreased,but blue value increased.Under the conditions that thermal effect time increased from 10 to 40 s at 90 ℃,the main changes of above factors happened at about 20 s,and after 20 s effect time the change of these factors was weak.These factors could reflect the form and progresses of zones of coagulation,injury of cutis and hypodermis.So OCT combined with reflection spectrum could provide a novel method that can be applied for the real-time,low-cost,in vivo and noninvasive optical biopsy on scald.

关 键 词：光学相干层析成像 可见近红外反射光谱技术 皮肤烫伤 小鼠模型 

分 类 号：O433.4[机械工程—光学工程]