脂联素对体外培养新生牛脂肪细胞ADPN、HSL mRNA表达的影响  

Adiponectin Effect of ADPN mRNA,Hormone-sensitive Lipase mRNA Expression on Primary Cultured Bovine Adipocytes

作　　者：于国健[1] 刘国文[1] 李小兵[1] 王哲[1] 李鹏[1,2] 黄海龙[1,3] 张燚[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [3]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国畜牧兽医》2011年第3期51-54,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30871897);吉林大学科学前沿与交叉学科创新项目(200903335)

摘　　要：取单层生长状态良好的犊牛脂肪细胞,分别添加0、2.5、5、15、30、50、100μg/mL外源牛重组脂联素(adiponectin,ADPN),培养12 h后提取总RNA,采用荧光定量PCR方法检测外源脂联素对脂肪细胞ADPN mRNA和HSL mRNA表达水平的影响。结果显示,随着ADPN添加量的增多,ADPN mRNA相对表达量呈下降趋势;添加ADPN 15μg/mL时,HSLmRNA的表达量达到峰值,之后呈下降趋势。结果表明体外培养的脂肪细胞中ADPN mRNA表达量受自身的负调节,而添加适量的ADPN可刺激脂肪细胞HSL mRNA的表达。This experiement on adipocyte of calf was adopt to make primary culture,select monolayer growth well adipocyte of calf,then add concentration of the additives was as ADPN： 0,2.5,5,15,30,50,100 μg/mL,after 12 h cultivation,abstract total RNA and test the effect of ADPN on the fat cell of ADPN mRNA and HSL mRNA expression by FQ-PCR method.With increasing the addition of ADPN,expression level of ADPN mRNA decreased;the expression level of HSL mRNA in fat cell was highest when the concentration of ADPN was 15 μg/mL in cell culture media,then decreased.The results showed as follows： the ADPN mRNA expression was regulated by negative regulator itself in adipocyte culture in vitro,but the appropriate amount concentration of ADPN could stimulate adipocyte HSL mRNA expression.

关 键 词：脂联素 脂肪细胞 ADPNmRNA HSLmRNA 

分 类 号：S852.2[农业科学—基础兽医学]