仔猪睾丸组织c-jun mRNA表达量与其血浆睾酮水平关系研究  

Study on the Relationship between c-jun mRNA Expression in Piglet's Testis and Serum Testosterone

作　　者：姚银竹[1] 肖榕[2] 吴群[1] 郑之琬[2] 卿丽娟[1] 徐茂森[1] 郑小波[2] 

机构地区：[1]西南大学动物科技学院,重庆400716 [2]西南大学荣昌校区动物医学系,重庆荣昌402460

出　　处：《中国畜牧兽医》2011年第3期139-143,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30270955);教育部重点科学技术项目(01106)

摘　　要：对不同时期仔猪血浆睾酮含量和睾丸组织c-jun mRNA表达量进行了检测,以初步推测c-jun mRNA表达与睾酮分泌的关系,为进一步体外研究原癌基因c-jun对睾酮分泌的影响奠定基础。试验选择1、3、5周龄仔猪作为研究对象,利用睾酮ELISA检测试剂盒检测血浆中睾酮含量;采用SYBR GreenⅠ实时荧光定量PCR检测睾丸组织c-jun mRNA的表达。结果表明,仔猪血浆睾酮水平随周龄增加而显著升高,其中3周龄时睾酮含量最高;c-jun在各周龄睾丸组织中均有表达,其中3周龄仔猪睾丸组织c-jun mRNA表达量最高。因此,仔猪睾酮分泌与c-jun mRNA表达量可能存在相关性。This study was established to explore the relationship between the expression of c-jun mRNA and testosterone secretion by comparing the c-jun mRNA expression in piglet＇s testis at different periods and its serum testosterone.1,3 and 5 weeks old pigs were selected.The concentrations of serum testosterone were detected by Testosterone ELISA Kit and the c-jun mRNA expressions were measured by using real-time PCR with SYBR GreenⅠ.As a result,the level of testosterone was gone up with the age and reached the highest point at 3 weeks old,c-jun expressed in each group,but the three weeks group had the highest expression.Therefore,there may be a dependability relationship between c-jun mRNA expression and testosterone secretion.

关 键 词：仔猪 睾酮 睾丸组织 c-junmRNA 实时荧光定量PCR 

分 类 号：Q57[生物学—生物化学]