归脾丸溶液对苯染毒小鼠学习记忆及海马形态学的影响  

Effect of Gui-pi Decoction on Learning Memory and Hippocampal Morphology in Mice Exposed to Benzene

作　　者：俞晓英[1] 徐厚谦[1] 刘立[1] 孙少伯[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《环境与职业医学》2011年第3期149-151,155,共4页Journal of Environmental and Occupational Medicine

基　　金：甘肃省2009年重大中医药科研项目(编号:GZK2009-08)

摘　　要：[目的]观察归脾丸溶液对苯中毒小鼠记忆功能的影响,初步揭示归脾丸溶液改善苯中毒小鼠记忆功能的机理。[方法]72只昆明小鼠,随机分为空白对照组、单纯染毒组、西药组、归脾丸溶液低(2 mg/kg.d)、中(4 mg/kg.d)、高(8 mg/kg.d)剂量组共6组,每组12只;西药组服用利血生。用Morris水迷宫系统进行行为学测定,并进行海马神经细胞形态学检查和外周血检测。[结果]与染毒组相比,归脾丸溶液高、中、低剂量组水迷宫定位导航寻找平台时间减少,差异均有统计学意义(P<0.01);归脾丸溶液高剂量组的水迷宫空间搜索跨台次数、目标区域游泳时间增加,差异有统计学意义(P<0.01或P<0.05)。海马组织中神经细胞形态及数量有明显差异。与西药组相比,归脾丸溶液高、中剂量组的血红蛋白明显升高,差异均有统计学意义(P<0.01或P<0.05)。[结论]归脾丸溶液可改善苯中毒小鼠的记忆功能。归脾丸溶液改善记忆功能可能与改善造血及海马神经元功能有关。[Objective] To observe the effect of Gui-pi decoction on memory function in mice exposed to benzene and explore possible mechanism of Gui-pi decoction in memory function improvement in benzene exposed mice.[Methods] Seventy two Kunming mice were randomly selected into 6 groups：the blank control group,the benzene exposure only group,the routine treatment group（Leucosonurn and Batilol）and the Gui-pi decoction groups in three doses（2,4 and 8 mg/kg·d）.Each group contained 12 mice.A Morris water maze systems was applied to study mice behavior.Neuronal morphology and blood tests were also performed.[Results] The time of platform navigation and searching in the morris water maze test were significantly lower in the Gui-qi decoction groups than the benzene exposure only group（P 0.01）.The number of cross-platform and swimming time in the target zone were significantly lower in the large dose Gui-pi group than in the benzene ezposure only group.The nerve cell morphology and count of hippocampus were significantly different.The hemoglobin were higher in the large and medium dose Gui-pi groups than in the routine treatment group（P 0.05）.[Conclusion] Gui-pi decoction may improve memory function in benzene exposed mice,which could contributed by improving hematopoiesis and hippocampal neurons function.

关 键 词：归脾丸溶液 学习记忆 血细胞异常 海马神经元 

分 类 号：R114[医药卫生—卫生毒理学]