海尔福治疗慢性氟中毒小鼠的实验研究  

Therapeutic Effect of Haierfu on Chronic Fluoride Poisoning in Mice

作　　者：覃淑云[1] 张树球[2] 

机构地区：[1]柳州医学高等专科学校.广西柳州545006 [2]右江民族医学院,广西百色533000

出　　处：《环境与职业医学》2011年第3期169-170,共2页Journal of Environmental and Occupational Medicine

基　　金：广西教育厅资助项目(编号:桂教科学1992年第1-32号)

摘　　要：[目的]研究海尔福对慢性氟中毒小鼠的治疗效果,为防治地方病氟中毒提供有效的药物。[方法]将30只健康小鼠随机分为3组,对照组、染毒组[100 mg/L氟化钠(NaF)]及治疗组(100 mg/L NaF+海尔福口服液)进行治疗。治疗后,通过测定血、股骨、粪便的氟含量,测定并分析肾组织上清液中脂质过氧化代谢产物:丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷光苷肽过氧化物酶(GSH-Px)的活性,以观察海尔福对氟中毒小鼠的治疗效果。[结果]血氟、骨氟含量:均以染毒组最高,分别是(5.68±1.42)mol/L、(2.97±1.92)mol/L;治疗组分别是(3.53±0.68)mol/L、(1.19±0.76)mol/L。染毒组与治疗组比较,差异有统计学意义(P<0.01);粪氟含量以治疗组最高(0.76±0.18)mol/L,与其他2组比较差异有统计学意义(P<0.01);肾组织中MDA含量、GSH-Px和SOD活性;中毒组[分别是(146.18±17.23)μmol/L、(100.23±16.34)×103 U/L、(92.35±7.83)U]与对照组[分别是(9.05±2.62)μmol/L、(379.08±10.02)×103 U/L、(342.04±13.65)U]及治疗组[分别是(72.46±5.83)μmol/L、(340.52±11.06)×103 U/L、(261.87±11.46)U]比较,差异均有统计学意义(P<0.01)。[结论]海尔福可能通过促进氟的排泄,在一定程度上恢复SOD、GSH-Px的活性,对慢性氟中毒小鼠有一定的治疗作用。[Objective] To study the effects of Haierfu on mice treated with fluoride and provide evidences forendemic fluorosis treatment.[Methods] A total of 30 mice were divided into 3 groups randomly：the control group,the model group（100 mg/L Naf）and the treated group（100 mg/L Naf＋ Haierfu oral solution）.After the treatement applied,observed histological changes,determined fluoride in blood,bone tissue and feces,as well as malony diadehyde（MDA）,superoxide dismutase（SOD）activity and glutathione peroxidase（GSH-Px）in kidney tissues.[Results] The fluorides in blood and bone tissue were the highest in the model group[（5.68±1.42）mol/L）and（2.97±1.92）mol/L,respectively],compared to the treated group[（3.53±0.68）mol/L and（1.19±0.76）mol/L）,P 0.01].The fluoride in feces was higher in the treated group（0.76±0.18）mol/L）,compared to the other groups,P 0.01.The MDA,the activities of SOD and GSH-Px in the kidney tissue of the model group were（146.18±17.23）μmol/L,（100.23±16.34）×103 U/L,（92.35±7.83）U,respectively;and（9.05±2.62）μmol/L,（379.08±10.02）×103 U/L and（342.04±13.65）U in the control group respectively and（72.46±5.83）μmol/L,（340.52±11.06）×103 U/L,（261.87±11.46）U in the treated group respectively.The model group showed higher values in these three items than the other two groups,P 0.01.[Conclusion] Haierfu could play some treating role to mice having fluorosis.

关 键 词：海尔福 氟中毒 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]