韦格纳肉芽肿病患者树突状细胞蛋白酶活化受体-2的表达  

Protease-activated receptor-2 expression on dendritic cell of Wegener＇s granulomatosis patients

作　　者：姜波[1] 华冰珠[1] 孙凌云[1] 

机构地区：[1]南京大学医学院附属鼓楼医院风湿免疫科,210008

出　　处：《中华风湿病学杂志》2011年第2期83-86,共4页Chinese Journal of Rheumatology

基　　金：江苏省科教兴卫工程项目（RC2007004）

摘　　要：目的 探索蛋白酶活化受体-2（PAR-2）在韦格纳肉芽肿病（WG）患者外周血免疫细胞特别是树突状细胞（DC）上的表达有无异常。方法 流式细胞仪检测健康人、活动期、非活动期、各治疗组WG患者外周血各免疫细胞、DC及DC样单核细胞表面PAR-2蛋白的表达。采用双尾Mann Whitney非参数t检验。结果 PAR-2在WG患者外周血中性粒细胞（PMN）、单核细胞、淋巴细胞等免疫细胞及DC上的表达与健康人差异无统计学意义，但在DC样单核细胞CD14^＋CD16^＋（t=3．823，P=0．004）和CD64^＋CD16^＋（t=2．652，P=0．024）单核细胞上的表达显著高于健康人。活动期WG患者外周血PMN（t=2．690，P=0．013）、单核细胞（t=1．668，P=0．047）、淋巴细胞（t=1．742，P=0．038）、BDCA3^＋DC（t=2．582，P=0．016）、CD11c^＋DC（t=1．828，P=0．044）和CD14^＋CD16^＋（t=3．419，P=0．002）和CD64^＋CD16^＋（t=3．494，P=0．005）单核细胞上PAR-2的表达显著高于非活动期患者。环磷酰胺和甲氨蝶呤治疗组WG患者上述细胞PAR-2的表达差异无统计学意义。结论 PAR-2在DC、DC样单核细胞及免疫细胞的表达与WG的发病发展相关。不同药物对PAR-2表达的影响差异无统计学意义。Objective The aim of this study was to assess the status of protease-activated receptor-2 （PAR-2） expression on the peripheral blood immune cells including dendritic cells （DC） of Wegener＇s granulo matosis （WG） patients. Methods Flow cytometry was used to analyze the expression of PAR-2 protein on peripheral blood immune cells, DC and DC-like monocytes of healthy controls （HC）, inactive, active and different medicine-treated WG patients. Two-tail Mann Whitney non-parametric test was used for statistical analysis. Results There was no difference of PAR-2 expression on PMN, monocytes, lymphoeytes and DC between WG patients and HC. However, PAR-2 expression on CD14^＋CD16^＋ （t=3.823, P=0.004） and CD64^＋ CD16^＋（t=2.652, P=0.024） DC-like monocytes was much higher in WG patients compared with HC. In active WG, expression of PAR-2 was up-regulated on the cell surface of PMN （t=2.690, P=0.013）, monocytes （t=1.688, P=0.047）, lymphoeytes （t=1.742, P=0.038）, BDCA3^＋DC （t=2.582, P=0.016）, CDlle＋DC （t= 1.828, P=0.044）, CD14^＋CD16^＋ （t=3.419, P=0.002） and CD64^＋CD^16＋（t=3.494, P=0.005） DC-like monoeytes when compared with inactive WG. PAR-2 expression on these cells was similar between eyclophosphamide （Cye） and Methotrcxate （MTX） treated WG patients. Conclusion PAR-2 expression on immune cells, DC and DC-like monoeytes correlates with WG occurrence and development. There is no difference in the effects on PAR-2 expression between various medications.

关 键 词：树突状细胞 韦格纳肉芽肿病 PAR-2 

分 类 号：R593.9[医药卫生—内科学]