流式细胞术分析外周血辅助性T17细胞在强直性脊柱炎患者外周血的表达及其意义  

Analysis of the expression and role of T helper 17 cells in the peripheral blood of patient with ankylosing spondylitis by flow cytometry

作　　者：李秀娟[1] 黄胜起[1] 郑辉[1] 陈卫光 李时英[1] 李仲笑[1] 韦峰[2] 

机构地区：[1]中山大学附属江门市中心医院检验科,529000 [2]中山大学附属江门市中心医院肾内科,529000 [3]广东省江门市新会区妇幼保健医院检验科

出　　处：《中华风湿病学杂志》2011年第2期116-118,共3页Chinese Journal of Rheumatology

基　　金：广东省医学科研基金

摘　　要：目的 检测强直性脊柱炎（AS）患者外周血辅助性T17（Th17）细胞的表达，探讨其在AS发病机制中的作用。方法 用流式细胞术检测20例As患者和15名健康对照者外周血Th17[白细胞介素（IL）-17]、Th1[干扰素（IFN）-r]、Th2（IL-4）细胞及人类白细胞抗原（HLA）-B27，同时检测红细胞沉降率（ESR）、C反应蛋白（CRP）等，并分析Th17细胞与Th1、Th2、HLA-B27及AS活动性指标的相关性。采用t检验和Spearman相关分析。结果 As患者外周血Th17细胞[（2．6±0．8）％]高于对照组[（1．1±0．4）％]（P〈O．01）；Th1（IFN-r）细胞[（3．9±0．8）％]低于对照组[（5．1±1．3）％]（P〈0．05）；Th2（IL-4）细胞[（4．1±1．6）％]比对照组[（3．1±1．4）％]升高，但差异无统计学意义（P〉0．05）；Th17细胞与HLA-B27百分数及与HLA-B27平均荧光强度、ESR、CRP无相关性（P〉O．05）；但是，Th17细胞表达水平有随HLA-B27百分数及HLA-B27平均荧光强度增加升高的趋势。结论 AS患者外周血Th1细胞减少，而Th17细胞增加，AS患者体内存在Th细胞的失衡，Th17比例变化是反映AS整个复杂免疫反应异常的一个重要环节。Objective To detect the expression of T helper 17 （Th17） cells in the peripheral blood of patient with ankylosing spondylitis （AS）, and discuss its role in the pathogenesis of AS. Methods Twenty AS patients and fifteen healthy controls were enrolled in the study. Th17 （IL-17）, Th1（IFN-r）, Th2（IL-4） cells and HLA-B27 of their peripheral blood were analyzed by flow cytometry, at the same time, the levels of ESR and CRP were also measured in order to analyze the relation of Th17 and HLA-B27, ESR as well as the CRP level. The statistical analysis was carried out with single-sample t-test and Speraman＇s correlation test. Results The level of Th17 cells was significantly higher in the perpipheral blood of AS patients [（2.6±0.8）%] than those in healthy controls [（ 1.1±0.4）% ] （P〈0.01）. The level of Th1 cells was significantly lower in the peripheral blood of AS patients [（3.9±0.8）%] than those in healthy controls [ （5.1±1.3）%] （P〈0.01） and the level of Th2 cells was not different in the peripheral blood of AS patients [（4.1±1.6）%] when compared to healthy controls [（3.1±1.4）%] （P〉0.05）. Th17 cells was not significantly correlated with the percentage and mean fluorescent intensity of HLA-B27, ESR, CRP （P〉0.05）; but there was a tendency thai increased expression level of Th17 cells was associated with elevated percentage and mean fluorescent intensity of HLA-B27. Conclusion The level of Th1 cells is decreased, but Th17 is increased in the peripheral blood of AS patients. Th cells are imbalance in AS, patients. The change of Th17 cells may be an important part of the uathogenesis of AS.

关 键 词：脊柱炎 强直性 T淋巴细胞 辅助诱导 流式细胞术 

分 类 号：R593.23[医药卫生—内科学]