日本新糠虾卵巢和肝胰腺主要细胞内5-HT的免疫组织化学定位  

The presence of 5-HT in ovary and hepatopancreas of Neomysis japonica using immunohistochemical method

作　　者：杨丽丽[1] 杨筱珍[1] 赵柳兰[1] 成永旭[1] 吴旭干[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海市高校水产养殖学E-研究院,上海201306

出　　处：《水产学报》2011年第2期208-213,共6页Journal of Fisheries of China

基　　金：国家自然科学资金项目(30700609);上海高校创新团队(第二期)项目;上海科委攻关项目(08dz1205904)

摘　　要：运用免疫组化方法,观察了5-HT阳性物质在日本新糠虾不同发育期的卵巢卵细胞和滤泡细胞及肝胰腺细胞中的分布和变化。结果表明:随卵细胞的发育,滤泡细胞逐渐迁移至其近旁。各期卵巢卵细胞和滤泡细胞及肝胰腺细胞中均存在大量5-HT阳性细胞,阳性物质呈深棕色;各期卵巢中5-HT在滤泡细胞内均呈阳性;在Ⅰ、Ⅱ和Ⅲ期卵巢中卵细胞胞质和胞核均呈阳性,且阳性强度呈递减;Ⅳ期卵巢中5-HT在卵细胞中呈阴性,而在Ⅴ期卵巢中5-HT在卵细胞中又呈现弱阳性。5-HT在发育各期的肝胰腺细胞中均呈阳性。同一期卵巢中,不同类型肝胰腺细胞间5-HT的分布没有差别。除Ⅳ期卵巢外,在其余各期卵巢中5-HT阳性强度在卵细胞胞质和肝胰腺细胞胞质中表现一致。The distribution of 5-HT in main cells of both ovary and hepatopancreas at different ovary stages of Neomysis japonica was observed and compared by using immunohistochemical method.These cells included follicule cell,oogonium,endogenous vitellogenic oocyte,exogenous vitellogenic oocyte,mature oocyte,embryonic cells,blister like cells,resorptive cells,fibrillar cells.The results showed that changes on the distribution of positive 5-HT cells were observed with ovary development.All the follicule cells are stained positively at each ovary stage.As for the oocyte,both the cytoplasm and nucleus are stained positively during the early developmental stages,including Stage Ⅰ,Ⅱ,and Ⅲ.But the positive intensity presents a negative trend with ovary development.The oocytes are stained negatively at Stage Ⅳ whereas they were found weak positive in Stage Ⅴ.In contrast,the hepatopancreas cells are stained positively during each stage.Moreover,there are no significant differences of positive 5-HT in different types of hepotapancreas cells during the ovary development.In addition,the 5-HT positive intensities of oocyte were consistent with hepatopancreas cytoplasm throughout all ovary developmental stages（except Stage Ⅳ）.

关 键 词：日本新糠虾 5-HT 卵细胞 滤细胞 肝胰腺细胞 

分 类 号：Q952[生物学—动物学] S917[农业科学—水产科学]