降钙素的镇痛作用及其对中脑导水管周围灰质中降钙素受体表达的影响  

Effect of calcitonin on analgesia and expression of calcitonin receptor in periaqueductal gray

作　　者：李树法[1] 向菲[1] 张韬威[1] 江超[2] 赵建勇[3] 张敏 梁愿[1] 陈兵[5] 

机构地区：[1]贵阳市第一人民医院内分泌科,550002 [2]贵阳市第一人民医院儿科,550002 [3]贵阳市第一人民医院外科,550002 [4]中华社区卫生服务中心 [5]第三军医大学附属西南医院内分泌科,重庆

出　　处：《中华内分泌代谢杂志》2011年第2期113-117,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：民政部“十一五”老年学科研项目资助（民人教科字[2008]47-1-48）

摘　　要：目的探讨鲑鱼降钙素的镇痛作用及其对中脑导水管周围灰质（PAG）中降钙素受体表达的影响。方法采用慢性压迫性损伤（CCI）的方法制备神经性疼痛的大鼠模型，热板实验评估热痛阈（TWL），von Frey纤丝测定机械痛阈（MNT），免疫组化法检测PAG中降钙素受体的分布，Western印迹检测降钙素受体蛋白的表达量。40只雄性SD大鼠被平均随机分配至5组：正常组、假手术组、CCI组、CCI＋鲑鱼降钙素皮下注射组和CCI＋鲑鱼降钙素PAG微量注射组。结果（1）假手术组大鼠TWL、MNT及PAG中降钙素受体表达较正常组无显著性差异（P〉0．05）；（2）CCI组大鼠TWL和MNT较正常组显著降低（P〈0．05），但降钙素受体表达显著高于正常组（P〈0．05）；（3）鲑鱼降钙素治疗后CCI大鼠的TWL、MNT和降钙素受体的表达均显著升高（P〈0．05），且PAG注射较皮下注射效果显著（P〈0．05）。结论鲑鱼降钙素能够提高CCI大鼠的痛阈并增加PAG中降钙素受体的表达量，而且PAG注射鲑鱼降钙素较皮下注射效果明显。Objective To investigate the analgesic effect of salmon calcitonin （sCT） and its effect on expression of calcitonin receptor （CT-R） in periaqueductal gray （PAG）. Methods Rat models of neuropathic pain were prepared by chronic constriction injury （CCI）. Thermal withdrawal latency （TWL） and mechanical nociceptive threshold （MNT） were measured using hot plate test and yon Frey monofilaments test. The distribution of CT-R in PAG was detected by immunohistochemical method. CT-R protein was quantitatively determined by western blotting. Fourty male SD rats were randomized into 5 groups： normal group, sham-CCI group, CCI group, CCI plus subcutaneous sCT group, and CCI plus microinjection of sCT into PAG group. Results TWL, MNT, and expression of CT-R in PAG showed no difference between normal group and sham-CCI group（ P〉0.05 ）. TWL and MNT in CCI group were significantly lower than those in normal group （ P〈0.05 ） , and expression of CT-R in CCI group Was significantly higher than that in normal group（ P〈0.05 ）. TWL, MNT and expression of CT-R in CCI rats increased significantly after sCT therapy（ P〈0.05 ）, and the effect was more marked in PAG injection group than subcutaneous injection group （ P〈0.05 ）. Conclusions sCT raises the pain threshold and increase the expression of CT-R in PAG of CCI rats, while PAG injection showed more marked effect than subcutaneous injection.

关 键 词：降钙素 镇痛 慢性压迫性损伤 中脑导水管周围灰质 降钙素受体 

分 类 号：R965[医药卫生—药理学]