外源乙烯及1-MCP对牡丹CTR基因表达的影响  

Effects of Ethylene and 1-MCP Treatments on the Expressions of CTR Genes of Tree Peony

作　　者：高娟[1] 贾培义[1] 王彦杰[1] 张超[1] 王玮然[1] 董丽[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术研究中心,北京100083

出　　处：《西北植物学报》2011年第1期19-26,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30972030);教育部博士点基金(20060022008)

摘　　要：采用RT-PCR法研究外源乙烯和1-MCP对牡丹品种洛阳红(Paeonia suffruticosa Luoyanghong)1级切花CTR基因家族3个成员基因表达的影响,以揭示乙烯在牡丹采后开花和衰老进程中的调控机制.结果表明,在花朵开放和衰老进程中,PsCTR1和PsCTR2类似组成型表达,PsCTR3随内源乙烯的增加表达增强.PsCTR2和PsCTR3表达受外源乙烯的促进,PsCTR1的表达仅在花朵开放后期受到外源乙烯的促进.1-MCP处理增加了PsCTR1和PsCTR2的表达,但对PsCTR3的表达起先促进后抑制的作用.复合处理的结果表明,1-MCP处理可以逆转乙烯处理对PsCTR1和PsCTR2的作用;在切花进入盛花期和衰老期后,乙烯处理可以逆转1-MCP处理对PsCTR1、PsCTR2和PsCTR3的作用.Using ethylene and 1-MCP treatment on the cut flowers of tree peony（Paeonia suffruticosa） ＇Luoyanghong＇ at stage 1,the effect of ethylene and 1-MCP on the expressions of CTR genes of ＇Luoyanghong＇ in opening and the senescence process were studied by RT-PCR.The results showed that during the flower opening and senescence processes,PsCTR1 and PsCTR2 were accumulated in a constitutive manner,while PsCTR3 was increased with increasing endogenous ethylene production.The expressions of both PsCTR2 and PsCTR3 were increased in response to exogenous ethylene.1-MCP up-regulated the expressions of PsCTR1 and PsCTR2.The results from the competitive treatment between ethylene and 1-MCP showed that the 1-MCP treatment could reverse the effect of ethylene in PsCTR1 and PsCTR2,but had nothing to do with PsCTR3,by contrast,the ethylene treatment could reverse the effect of 1-MCP in PsCTR1,PsCTR2 and PsCTR3 only when the flowers arrived full opening or senescing stage.

关 键 词：CTR1 乙烯 切花 牡丹 

分 类 号：Q789[生物学—分子生物学]