青海省小麦品种中Yr10和Yr15基因及其1BL/1RS易位的分子检测  

Molecular Detection of Yr10,Yr15 Genes and 1BL/1RS Translocation in Qinghai Wheat Cultivars

作　　者：王欣[1,2,3] 张怀刚[1,2] 刘宝龙[1,2] 张波[1,2,3] 柳觐[1,2,3] 刘琦[1,2,3] 刘登才[1,2] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810001 [2]中国科学院高原生物适应与进化重点实验室,西宁810001 [3]中国科学院研究生院,北京100049

出　　处：《西北植物学报》2011年第1期57-63,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-YW-N-052);中国科学院知识创新工程重大项目(KSCX1-YW-03);国家科技支撑计划课题(2007BAD52B08);青海省重点科技攻关(2006-N-137)

摘　　要：利用抗条锈病基因Yr10和Yr15的SCAR和Barc8标记以及1BL/1RS易位的复合标记,对青海省育成和引进的137份小麦品种进行检测,以明确Yr10和Yr15基因以及1BL/1RS易位在青海小麦品种资源中的分布.结果显示:在137份材料中,有4份检测到Yr10基因,19份检测到Yr15基因,分别占参试材料的2.9%和13.9%,没有检测到同时携带Yr10和Yr15基因的材料;有22份材料为1BL/1RS易位,占参试材料的16.1%.研究表明,青海省大部分小麦抗锈品种及1BL/1RS易位品种为外引种品种.137 wheat cultivars bred and imported in Qinghai Province were detected for stripe rust resistant genes Yr10,Yr15 and 1BL/1RS translocation by special molecular markers（SCAR,Barc8,Glu-B3 and Sec-P1,P2）.The aims of this study were to find out the distributions of these genes and 1BL/1RS translocation in Qinghai wheat cultivars.The results showed that among the 137 materials,there were only 4 cultivars with Yr10 accounting for 2.9%,19 cultivars with Yr15 accounting for 13.9% and 22 cultivars with 1BL/1RS translocation accounting for 16.1%.No material was found containing both Yr10 and Yr15 genes.And most of those cultivars tested with Yr10,Yr15 and 1BL/1RS translocation in Qinghai were introduced from other provinces and abroad.

关 键 词：小麦 分子标记 抗条锈病基因 1BL/1RS易位系 

分 类 号：Q343.24[生物学—遗传学]