风信子品种间杂交与幼胚离体培养研究  

Study on the Hybrid Hyacinthus orientalis Varieties and the Hybrid Embryo in vitro Culture

作　　者：李玉萍[1] 王春彦[1] 罗凤霞[1] 韩美[1] 

机构地区：[1]金陵科技学院园艺学院,南京210038

出　　处：《西北植物学报》2011年第1期186-191,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家948项目(2009-4-12);金陵科技学院基金(JIT-RCYJ-2007001)

摘　　要：选用5个风信子品种进行花粉生命力测定,对完全双列杂交获得的杂种种子剥胚进行离体培养.试验表明:(1)风信子不同品种花粉萌发率差别较大,其中Fondante的花粉萌发率最高为74.45%,Amsterdam的最低为12.68%,其他3个品种的花粉萌发率介于二者之间.(2)品种间自交杂交亲和力存在一定差异,结实率大于30%的组合从高到低依次为Fondante×Atlantic(61.2%)、Fondante×Fondante(54.0%)、Carnegie×Carnegie(42.6%)、JanBos×JanBos(36.0%)、Carnegie×Atlantic(32.7%)、Atlantic×Fondante(30.0%),组合Atlantic×Amster-dam、Carnegie×JanBos、Amsterdam×Fondante、Amsterdam×JanBos、JanBos×Amsterdam未得到果实,其余组合结实率介于0～30%之间.(3)适宜风信子杂交幼胚离体培养的初代培养基为MS+100 mg/L谷氨酰胺,其次为MS+0.2 mg/LIBA+0.02 mg/L6-BA+100 mg/L谷氨酰胺和MS+0.5 mg/LIBA+0.05 mg/L6-BA,它们的幼胚平均成活率分别为91.33%、84.00%和77.87%.5 Hyacinthus orientalis varieties were selected to carry on the pollen vitality determination,the complete diallel crossing and culture the hybrid young embryo in vitro.The result showed：（1）A significant difference in pollen germination rate was found among various varieties,and the highest variety of pollen germination rate was Fondante（74.45%）,and the lowest was Amsterdam,only 12.68%,and the other varieties were between the two;（2）Variety inbred line hybrid affinity existed certain difference.The combinations with the fruit rate more than 30% were Fondante×Atlantic（61.2%）,Fondante×Fondante（54.0%）,Carnegie×Carnegie（42.6%）,JanBos×JanBos（36.0%）,Carnegie×Atlantic（32.7%）,Atlantic×Fondante（30.0%）,the combinations Atlantic×Amsterdam,Carnegie×JanBos,Amsterdam×Fondante,Amsterdam×JanBos,JanBos×Amsterdam didn＇t obtain the fruit and other combinations were between 0～30%.（3）The suitable initially generation culture medium of H.orientalis hybridizes young embryo was MS＋valley ammonia amide 100 mg/L,MS＋0.5 mg/L IBA＋0.05 mg/L 6-BA and MS＋0.2 mg/L IBA＋0.02 mg/L 6-BA＋the valley ammonia amide 100 mg/L next bests,and the average survival rates of young embryos were 91.33%,84.00% and 77.87%.

关 键 词：风信子 杂交组合 杂种胚 离体培养 

分 类 号：Q813.1[生物学—生物工程]