牙周膜细胞在碱处理后的多孔钛片表面的活性  

Activity of human periodontal ligament cell on the surface of porous titanium after alkali treatment

作　　者：刘君瑜[1] 刘艳 汪永跃[1] 傅云婷[3] 

机构地区：[1]四川大学华西口腔医院种植科,成都610041 [2]第四军医大学口腔医院种植科,西安710032 [3]深圳市龙岗中心医院口腔科,深圳518116

出　　处：《国际口腔医学杂志》2011年第2期154-158,共5页International Journal of Stomatology

摘　　要：目的以碱处理和仿生溶液在多孔钛表面制备类骨磷灰石涂层,观察人牙周膜细胞(PDLC)在碱处理前后多孔钛表面的黏附生长,评价多孔钛类骨磷灰石表面的生物相容性。方法松装烧结法制备多孔钛片,60℃下行碱处理,在模拟体液中沉积类骨磷灰石涂层。扫描电镜和X射线衍射仪分析涂层表面形貌、物相和结构,能量散射光谱测定仪分析其表面成分,扫描电镜下观察PDLC在碱处理前后钛片表面的生长形态。甲噻唑四唑氮比色检测细胞活性,进行细胞毒性评价。结果经碱处理后,多孔钛在仿生溶液中可形成一层均匀的类骨磷灰石涂层,该涂层可促进PDLC增殖。结论多孔钛表面类骨磷灰石涂层具有良好的生物相容性,是PDLC的良好生长载体。Objective To observe the growth of human periodontal ligament cell（PDLC） attaching on the surfaces of porous titanium before or after alkali treatment： To evaluate the biocompatibility of bone-like apatite. Methods Porous titanium which was made by loosening sintering was cut into pieces.Bone-like apatite formation occured on the pieces of porous titanium in the SBF after alkali treatment at 60 ℃.The morphology of the obtained coating was observed by scanning electric microscope（SEM）.The phase and structure of the coating were analyzed by X-ray diffraction.The chemical composition of the coating was analyzed by energy-dispersive spectrometry.The morphology of PDLC was observed by SEM.The cell activity was monitored with methyl thiazolyl tetrazolium assay and cytotoxicity of porous titanium was evaluated.Results After alkali treatment,uniform coating occured on the surface of porous titanium in the SBF.The coating promoted proliferation of PDLC.Conclusion Bone-like apatite has a good biocompatibility and is a suitable carrier for PDLC.

关 键 词：多孔钛 类骨磷灰石 牙周膜细胞 

分 类 号：R318.08[医药卫生—生物医学工程]