补肾壮骨中药对成骨质量和骨钙蛋白的影响  

Effect of Chinese tradition medicine on the quality of new bone and expression of osteocalcin

作　　者：闫宝勇[1] 董福生[2,3] 董玉英[2,3] 郝福良[2,3] 张扬 陈爱哲 

机构地区：[1]河北医科大学第一医院口腔颌面外科 [2]河北医科大学口腔医院口腔颌面外科 [3]河北省口腔医学重点实验室,石家庄050017

出　　处：《国际口腔医学杂志》2011年第2期159-164,170,共7页International Journal of Stomatology

基　　金：河北省自然科学基金资助项目(C2007000814);河北省科学技术研究与发展指令计划基金资助项目(06276102D-111)

摘　　要：目的探讨补肾壮骨中药对下颌骨牵张成骨的作用机制。方法将16只英国小猎兔犬(beagle)随机分为中药组和对照组并行其右侧下颌骨劈开,植入常规组合式骨牵张器,经7d延迟后,以0.5mm每12h的速度连续牵张10d。中药组自牵张器植入术后第1天,口服补肾壮骨中药至试验结束。分别于固定后的1、2、3、4周,每组处死小猎兔犬2只,留取标本后行组织学观察、骨组织计量学分析和骨钙蛋白检测。结果 1)大体标本:在第1～2周时,两组大体标本无明显差异,牵张间隙处骨膜增厚,牵张间隙有轻微的动度;第3周时,两组标本增生的骨膜变薄,两骨断端动度消失;第4周时,两组骨膜厚度逐渐恢复到正常骨膜的厚度,两切骨断端已难以辨认。中药组牵张间隙处骨组织较致密且光滑。2)组织形态学改变:在第1周时,牵张沟少量区域充满蜂窝状的结缔组织,在接近于切开的近髓腔处有膜状新骨形成;第2周时,牵张间隙内由中央向两侧,依次有纤维区带、指状类骨质区带和新生骨小梁区带;第3周时,牵张沟内大部分区域有新骨形成,中药组成骨细胞的数量较多且较活跃,新生骨小梁区带较宽,而纤维区带较窄;中药组牵张间隙内新骨形成活跃,新生骨小梁粗大,新生骨组织也较为成熟;第4周时,牵张间隙3个区带消失,代之以近似平行排列的新生骨小梁;中药组骨小梁排列密集,形态粗大且均匀,而对照组骨小梁则稀疏且粗细不均。3)骨组织计量学分析:在第1～2周时,中药组骨小梁的面积、周长和面密度与对照组相比较无明显差异。在第3～4周时,中药组骨小梁的面积、周长和面密度均高于对照组。4)骨钙蛋白的mRNA检测:骨钙蛋白mRNA主要表达于成骨细胞的细胞质和新生骨的表面。第1周时,中药组骨钙蛋白mRNA的表达量与对照组相比较,无明显差异。在第2、3、4周时,中药组骨钙蛋白mRNA的表达量均高于对照组。�Objective To investigate the effect of Chinese tradition medicine on mandibular distraction osteogenesis.Methods Sixteen beagles were randomly divided into medicine group and control group.A customized component distraction device was applied to the right side of mandible,and the transverse osteotomy was performed in all animals.After 7 days,distraction was started at the rate of 0.5 mm each 12 h and continued for 10 days.The beagles in medicine group drank Chinese tradition medicine from the first day after postoperation to the last of the trial.2 animals were killed at week 1,2,3,and 4 after consolidation.The mandibles were harvested to examine the distraction tissues histologically,bone histomorphontry measures and detect of osteocalcin（OC）.Results 1）Observation of the general specimens： During the 1-2 weeks of the fixed period,no significant difference was shown between the two groups.The distraction gap was covered with a thick layer of collagen connective tissue,and there was a slight movement of distraction gap.In the 3 weeks,from the two group samples,the proliferated periosteal get thinner,and the movement of two bone stumps disappeare could be observed.After the 4 weeks,the thickness of the periosteum at distraction gap of the two groups gradually returned to normal.It was difficult to identify the two-cut stump of the bone.New bone tissue of the medicine group was better than that of the control group,and bone tissue at the distraction gap was more dense and smooth.2）Histomorphological changes：In the 1st and the 2 weeks of the fixed period,there was no significant difference between the two groups.In the 1st week,a small part of distraction zone was filled with honeycomb connective tissue,and close to the nearly cut of marrow department,new membranous bone formed.In the 2 weeks,the distraction gap from the center to both sides,showed three clear bands： The fiber zone,finger osteoid zone and newborn trabecular zone.In the 3 weeks,new bone formation can be seen in most of the distraction re

关 键 词：小猎兔犬 补肾壮骨中药 骨钙蛋白 牵张成骨 骨组织计量学 

分 类 号：R285.5[医药卫生—中药学]