外源性血管内皮生长因子对大鼠损伤脊髓神经元的作用  

Effect of exogenous vascular endothelial growth factor on neurons in injured spinal cord in adult rats

作　　者：关永林[1] 李盛华[1] 李晨旭[1] 杨永清[2] 郑祖根 

机构地区：[1]甘肃省中医院骨科,730000 [2]兰州大学第二医院骨科,730030 [3]苏州大学第二附属医院骨科,215006

出　　处：《中国神经免疫学和神经病学杂志》2011年第2期114-118,共5页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的研究外源性血管内皮生长因子（VEGF）局部应用对大鼠脊髓损伤（SCI）后脊髓灰质神经元的作用。方法采用改良Allen氏重量打击法建立大鼠急性SCI模型,将72只模型大鼠随机分为VEGF治疗组〔人工基底膜（Matrigel蛋白胶）10μL＋重组人VEGF1650.2μg〕、基底膜对照组（Matrigel蛋白胶10μL）和空白对照组（生理盐水10μL）3组,分别经蛛网膜下腔给药。于SCI后1、2、3、4周分别观察模型鼠脊髓横切面灰质神经元数量、神经元截面积和尼氏体密度恢复情况。结果 SCI后1~4周VEGF治疗组脊髓横切面灰质神经元数量（分别为76.34±6.25、108.25±11.74、144.33±13.22、165.96±18.11）和神经元截面积〔分别为（177.87±14.42）（、229.63±21.74）（、340.55±32.66）（、390.30±46.19）μm2〕均高于同期基底膜对照组〔神经元数量分别为40.89±7.20、50.61±13.11、59.78±9.46、80.13±10.84,神经元截面积分别为（108.98±15.03）（、148.53±18.38）、（163.22±16.77）（、208.87±29.58）μm2〕和空白对照组〔神经元数量分别为37.66±6.69、52.36±5.17、60.58±9.13、78.56±13.56,神经元截面积分别为（114.55±14.22）（、139.24±15.17）、（157.11±20.26）（、185.23±21.65）μm2〕（P〈0.01,P〈0.05）。SCI后1周时VEGF治疗组神经元尼氏体密度（0.2089±0.0210）与基底膜对照组（0.2012±0.0145）、空白对照组（0.1982±0.0106）比较差异均无统计学意义（P〉0.05）,SCI后2、3、4周,VEGF治疗组尼氏体密度（分别为0.2489±0.0217、0.2908±0.0327、0.3508±0.0362）亦大于基底膜对照组（分别为0.2198±0.0214、0.2406±0.0198、0.2605±0.0322）和空白对照组（分别为0.2044±0.0231、0.2356±0.0311、0.2582±0.0245）（均P〈0.05）。两对照组各指标均无统计学差异。结论局部应用外源性VEGF可能对SCI后大鼠脊髓灰质神经元具有保护作用。Objective To investigate the effects of local application of exogenous vascular endothelial growth factor（VEGF） on spinal cord neurons after spinal cord injury（SCI） in adult rats.Methods The contusion injury in spinal cord was induced by using the modulated Allen＇s weight drop method.Seventy-two rats were randomly divided into 3 groups,VEGF-treated group and two control groups.In the VEGF-treated animals,human recombinant VEGF165（0.2 μg）,mixed with Matrigel（10 μL）,which was a reconstituted membrane basement protein extract,was injected into subrachnoid space.One control group received 10 μL matrigel alone（the matrigel control group）,and another received 10 μL saline（the blank control group）.1,2,3 and 4 weeks after spinal cord injury,animals were collected.The number of neurons,the cross section area of neurons and the recovery of the density of the Nissls＇ body were evaluated.Results VEGF-treated animals had an increased amount of spared tissue in the lesion area.The number of neurons（76.34±6.25,108.25±11.74,144.33±13.22,165.96±18.11,respectively）,the cross section area of neurons [（177.87±14.42）,（229.63±21.74）,（340.55±32.66）,（390.30±46.19）μm2,respectively] in the VEGF-treated experimental group were much better than those in the matrigel control group [The number of neurons： 40.89±7.20,50.61±13.11,59.78±9.46,80.13±10.84,respectively;the cross section area of neurons：（108.98±15.03）,（148.53±18.38）,（163.22±16.77）,（208.87±29.58）μm2,respectively] and blank control group [The number of neurons： 37.66±6.69,52.36±5.17,60.58±9.13,78.56±13.56,respectively;the cross section area of neurons：（114.55±14.22）,（139.24±15.17）,（157.11±20.26）,（185.23±21.65）μm2,respectively] at all time points after SCI（P0.01,P0.05）.At one week after SCI,there was no significant difference in the density of the Nissls＇ body among the VEGF-treated experimental group（0.2089±0.0210）,the matrigel control group（0.2012±0.014

关 键 词：脊髓损伤 血管内皮生长因子 神经元 

分 类 号：R651.2[医药卫生—外科学]