微囊化异种嗅球组织移植联合β-七叶皂甙钠对脊髓损伤大鼠血脊髓屏障功能和血管源性脊髓水肿的影响  

Effects of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium on blood-spinal barrier function and vasogenic spinal cord edema in spinal cord injury rats

作　　者：王航辉[1] 张博[2] 朱文侠[1] 李小记[1] 韩振奎[1] 王璐[1] 赵琳[1] 刘伯锋[1] 

机构地区：[1]延安大学医学院解剖学教研室,陕西延安716000 [2]湖州师范学院医学院解剖学教研室,浙江湖州313000

出　　处：《四川医学》2011年第3期301-304,共4页Sichuan Medical Journal

基　　金：陕西省卫生厅科学研究基金项目资助(编号:2010H25);陕西省自然科学基金资助项目(编号:08JK502);延安市科技计划项目资助(编号:2009KS-22);延安大学专项科研基金资助项目(编号:YD2008-39)

摘　　要：目的旨在探讨微囊化异种嗅球组织移植联合β-七叶皂甙钠对脊髓继发性损伤的治疗保护作用及机制。方法健康SD大鼠,随机分为损伤对照组、微囊化兔嗅球组织移植组、微囊化兔嗅球组织移植联合β-七叶皂甙钠治疗组,损伤组又设1、37、1、42、8d 5个时相点,紫外分光光度计检测脊髓伊文氏蓝含量,干湿重法测定脊髓组织含水量;同时电镜观察血脊髓屏障的变化;神经功能评分法(BBB法)评价大鼠术后运动功能的恢复情况。结果脊髓损伤后脊髓伊文氏蓝含量增加、脊髓血管源性水肿,大鼠的运动功能缺失,经微囊化异种嗅球组织移植联合β-七叶皂甙钠治疗后血脊髓屏障通透性降低、水肿明显减轻,大鼠的运动功能恢复。结论微囊化异种嗅球组织移植对脊髓继发性损伤有较好的疗效,联合应用嗅球组织细胞和β-七叶皂甙钠在脊髓损伤修复治疗中具有协同作用。Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium in rats at the early stages of spinal cord injury.Methods Healthy SD rats were divided into three groups as following： injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium group,the later two groups were quantified at 1,3,7,14,and 28 d post spinal cord injury.Spinal Evans blue content was examined with the ultraviolet spectrophotometer;Spinal cord water content was detected by caculating dry/wet weight.The ultrastructural change of blood-spinal barrier was observed by transmission electron microscope.BBB score was designed to assess its behavioral ability.Results Evans blue content were significantly increased,vasogenic spinal cord edema,and loss of motor function in rats after spinal cord injury.Permeability of blood-spinal barrier and vasogenic spinal cord edema were obviously decreased,motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium.Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent.There may be a synergistic effect between olfactory ensheating cells and β-aescinate sodium.

关 键 词：脊髓损伤 异种移植 嗅鞘细胞 Β-七叶皂甙钠 

分 类 号：R651.2[医药卫生—外科学]