硝普钠缓解花生铝毒害作用和影响AhSAG和AhBI-1基因表达  

Effects of SNP on AhSAG and AhBI-1 Genes Expression and Amelioration of Aluminum Stress to Peanut (Arachis hypoganea L.)

作　　者：詹洁[1,2] 王天菊[1] 何虎翼[1] 李创珍[1] 何龙飞[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西大学国防教育学院,广西南宁530004

出　　处：《作物学报》2011年第3期459-468,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30960181;30560070);广西大学科研基金(XJZ100341)资助

摘　　要：以花生为材料,研究了铝胁迫诱导花生发生细胞程序性死亡的条件下,加入外源NO供体硝普纳(SNP)对不同耐性花生品种根尖生长及相关细胞程序性死亡基因的影响。根长生长、根尖苏木精染色和铝含量测定结果表明,适当浓度的SNP能够缓解一定浓度铝对花生根生长的毒害作用,1.0mmolL-1的SNP缓解作用最明显,此浓度下,99-1507和中花2号与对照相比,根长分别增加45%和52%,根尖铝含量分别降低10%和23%,苏木精染色变浅。加入1.0mmolL-1的SNP处理后,与对应铝胁迫相比,花生衰老基因AhSAG的相对表达量在耐铝品种99-1507中先升高后降低,0.1mmolL-1和0.4mmolL-1Al3+处理时显著下降;在铝敏感品种中花2号中呈缓慢升高的趋势,0.1mmolL-1和0.4mmolL-1Al3+处理时显著上升。花生细胞程序性死亡相关基因AhBI-1的相对表达量在99-1507中呈先升高后降低的趋势,在中花2号中则先降低后升高,当99-1507在0和0.02mmolL-1Al3+处理时,中花2号在0、0.02和0.10mmolL-1Al3+处理时,SNP处理后的AhBI-1表达量均低于对应的单独铝处理。由此说明,NO对花生铝毒害有一定的缓解作用,其作用机理可能与影响细胞程序性死亡基因表达而调控细胞程序性死亡有关。Nitric oxide（NO）plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plant kingdom.Aluminum（Al）toxicity is a major constraint for crop production in acidic soils worldwide,especially in southern China.Using sodium nitroprusside（SNP）as the NO donor,we evaluated the effect of NO on root elongation and the expression levels of related genes in peanut seedlings under different Al 3＋ stress.The results showed that exogenous NO in proper concentration could alleviate Al toxicity in root tips of peanut,especially at 1.0 mmol L-1SNP.SNP at 1.0 mmol L-1enhanced root growth of 99-1507（Al-resistant）and Zhonghua 2（Al-sensitive）by 45%and 52%,respectively.After SNP（1.0 mmol L-1）treatment,the Al 3＋ contents in root tips were decreased by 10%and 23%in both cultivars,respectively.Simultaneously,the color of root tips dyed with hematoxylin became lighter.Compared to Al stress only,the relative expression levels of AhSAG gene in 99-1507 under 0.1 and 0.4 mmol L-1 Al 3＋ were increased at begining and then decreased significantly after adding SNP.In Zhonghua 2,the expressions of AhSAG gene appeared in slow increases when SNP（1.0 mmol L-1）was added,and the increases were significant under 0.1 and 0.4 mmol L-1 Al 3＋ treatments.Compared with Al stress only,the relative expression level of programmed cell dealth（PCD）-related gene,AhBI-1,showed an increase-decrease trend in 99-1507 and a reverse change in Zhonghua 2 after SNP was added at 1.0 mmol L-1.The relative expressions of AhBI-1 gene were lower in the Al＋SNP treatments than in the single Al treatments at Al3 ＋concentrations of 0 and 0.02 mmol L-1in 99-1507,whereas they were lower in the Al＋SNP treatments than in the single Al treatments at Al 3＋ concentrations of 0,0.02,and 0.1 mmol L-1 in Zhonghua 2.The results showed that NO can alleviate Al toxicity in peanut,and the possible mechanism is that NO regulates the expression levels of PCD-related genes.

关 键 词：一氧化氮 花生 铝胁迫 缓解效应 基因表达 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]